Detailed information NTG0435816

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IMO23_RS04295
• Genome accession: NZ_CP062919
• Coordinates: 895923..897041 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A086HYC5
• Organism: Acinetobacter baumannii strain Res13-Abat-PEA21-P4-01-A
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 890923..902041

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IMO23_RS04265 (IMO23_04265)	-	891752..892600 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
IMO23_RS04270 (IMO23_04270)	-	892853..893308 (+)	456	WP_000782976.1	bacteriohemerythrin	-
IMO23_RS04275 (IMO23_04275)	-	893461..894528 (+)	1068	WP_195707712.1	hypothetical protein	-
IMO23_RS04280 (IMO23_04280)	-	894528..894851 (+)	324	WP_000442589.1	RnfH family protein	-
IMO23_RS04285 (IMO23_04285)	-	894906..895304 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
IMO23_RS04290 (IMO23_04290)	fur	895416..895853 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
IMO23_RS04295 (IMO23_04295)	pilU	895923..897041 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IMO23_RS04300 (IMO23_04300)	pilT	897069..898106 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
IMO23_RS04305 (IMO23_04305)	-	898233..898925 (+)	693	WP_001108520.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=435816 IMO23_RS04295 WP_000347036.1 895923..897041(-) (pilU) [Acinetobacter baumannii strain Res13-Abat-PEA21-P4-01-A]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=435816 IMO23_RS04295 WP_000347036.1 895923..897041(-) (pilU) [Acinetobacter baumannii strain Res13-Abat-PEA21-P4-01-A]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A086HYC5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392