Detailed information NTG0433682

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HBN30_RS14495
• Genome accession: NZ_CP050526
• Coordinates: 3017108..3018226 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain VB2139
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3012108..3023226

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HBN30_RS14485 (HBN30_14485)	-	3015224..3015916 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
HBN30_RS14490 (HBN30_14490)	pilT	3016043..3017080 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
HBN30_RS14495 (HBN30_14495)	pilU	3017108..3018226 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HBN30_RS14500 (HBN30_14500)	fur	3018296..3018733 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
HBN30_RS14505 (HBN30_14505)	-	3018845..3019243 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
HBN30_RS14510 (HBN30_14510)	-	3019298..3019621 (-)	324	WP_000442587.1	RnfH family protein	-
HBN30_RS14515 (HBN30_14515)	-	3019621..3020688 (-)	1068	WP_002019572.1	hypothetical protein	-
HBN30_RS14520 (HBN30_14520)	-	3020841..3021296 (-)	456	WP_000782976.1	bacteriohemerythrin	-
HBN30_RS14525 (HBN30_14525)	-	3021549..3022397 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=433682 HBN30_RS14495 WP_000347039.1 3017108..3018226(+) (pilU) [Acinetobacter baumannii strain VB2139]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=433682 HBN30_RS14495 WP_000347039.1 3017108..3018226(+) (pilU) [Acinetobacter baumannii strain VB2139]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395