Detailed information NTG0433635

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HBM81_RS14735
• Genome accession: NZ_CP050523
• Coordinates: 3046832..3047950 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain VB7036
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3041832..3052950

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HBM81_RS14725 (HBM81_14725)	-	3044948..3045640 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
HBM81_RS14730 (HBM81_14730)	pilT	3045767..3046804 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
HBM81_RS14735 (HBM81_14735)	pilU	3046832..3047950 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HBM81_RS14740 (HBM81_14740)	fur	3048020..3048457 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
HBM81_RS14745 (HBM81_14745)	-	3048569..3048967 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
HBM81_RS14750 (HBM81_14750)	-	3049022..3049345 (-)	324	WP_000442587.1	RnfH family protein	-
HBM81_RS14755 (HBM81_14755)	-	3049345..3050412 (-)	1068	WP_002019572.1	hypothetical protein	-
HBM81_RS14760 (HBM81_14760)	-	3050565..3051020 (-)	456	WP_000782976.1	bacteriohemerythrin	-
HBM81_RS14765 (HBM81_14765)	-	3051273..3052121 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=433635 HBM81_RS14735 WP_000347039.1 3046832..3047950(+) (pilU) [Acinetobacter baumannii strain VB7036]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=433635 HBM81_RS14735 WP_000347039.1 3046832..3047950(+) (pilU) [Acinetobacter baumannii strain VB7036]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395