Detailed information NTG0432629

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HB368_RS14875
• Genome accession: NZ_CP050425
• Coordinates: 3073868..3074986 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347035.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain PM194188
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3068868..3079986

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HB368_RS14865 (HB368_14865)	-	3071984..3072676 (-)	693	WP_001108510.1	YggS family pyridoxal phosphate-dependent enzyme	-
HB368_RS14870 (HB368_14870)	pilT	3072803..3073840 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
HB368_RS14875 (HB368_14875)	pilU	3073868..3074986 (+)	1119	WP_000347035.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HB368_RS14880 (HB368_14880)	fur	3075056..3075493 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
HB368_RS14885 (HB368_14885)	-	3075605..3076003 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
HB368_RS14890 (HB368_14890)	-	3076058..3076381 (-)	324	WP_000442585.1	RnfH family protein	-
HB368_RS14895 (HB368_14895)	-	3076381..3077448 (-)	1068	WP_005120426.1	hypothetical protein	-
HB368_RS14900 (HB368_14900)	-	3077601..3078056 (-)	456	WP_034129532.1	bacteriohemerythrin	-
HB368_RS14905 (HB368_14905)	-	3078309..3079157 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41821.98 Da
• Isoelectric Point: 6.5675

>NTDB_id=432629 HB368_RS14875 WP_000347035.1 3073868..3074986(+) (pilU) [Acinetobacter baumannii strain PM194188]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDSQ

Nucleotide

• Length: 1119 bp

>NTDB_id=432629 HB368_RS14875 WP_000347035.1 3073868..3074986(+) (pilU) [Acinetobacter baumannii strain PM194188]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGCGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACAGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.817	100	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39