Detailed information NTG0432412

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HB366_RS14375
• Genome accession: NZ_CP050412
• Coordinates: 2998453..2999571 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain PM192696
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2993453..3004571

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HB366_RS14365 (HB366_14365)	-	2996569..2997261 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
HB366_RS14370 (HB366_14370)	pilT	2997388..2998425 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
HB366_RS14375 (HB366_14375)	pilU	2998453..2999571 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HB366_RS14380 (HB366_14380)	fur	2999641..3000078 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
HB366_RS14385 (HB366_14385)	-	3000190..3000588 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
HB366_RS14390 (HB366_14390)	-	3000643..3000966 (-)	324	WP_000442587.1	RnfH family protein	-
HB366_RS14395 (HB366_14395)	-	3000966..3002033 (-)	1068	WP_002019572.1	hypothetical protein	-
HB366_RS14400 (HB366_14400)	-	3002186..3002641 (-)	456	WP_000782976.1	bacteriohemerythrin	-
HB366_RS14405 (HB366_14405)	-	3002894..3003742 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=432412 HB366_RS14375 WP_000347039.1 2998453..2999571(+) (pilU) [Acinetobacter baumannii strain PM192696]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=432412 HB366_RS14375 WP_000347039.1 2998453..2999571(+) (pilU) [Acinetobacter baumannii strain PM192696]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395