Detailed information NTG0432385

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: HB366_RS01455
• Genome accession: NZ_CP050412
• Coordinates: 311491..312549 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain PM192696
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 306491..317549

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HB366_RS01440 (HB366_01440)	-	307596..307928 (+)	333	WP_000169594.1	hypothetical protein	-
HB366_RS01445 (HB366_01445)	-	307931..308755 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
HB366_RS01450 (HB366_01450)	ponA	308773..311328 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
HB366_RS01455 (HB366_01455)	pilM	311491..312549 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
HB366_RS01460 (HB366_01460)	pilN	312549..313190 (+)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
HB366_RS01465 (HB366_01465)	pilO	313187..313927 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
HB366_RS01470 (HB366_01470)	pilP	313938..314465 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
HB366_RS01475 (HB366_01475)	pilQ	314533..316698 (+)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
HB366_RS01480 (HB366_01480)	aroK	316710..317279 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=432385 HB366_RS01455 WP_002014066.1 311491..312549(+) (pilM) [Acinetobacter baumannii strain PM192696]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=432385 HB366_RS01455 WP_002014066.1 311491..312549(+) (pilM) [Acinetobacter baumannii strain PM192696]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423