Detailed information NTG0432365

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HB365_RS14220
• Genome accession: NZ_CP050410
• Coordinates: 2983032..2984150 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain PM1912235
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2978032..2989150

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HB365_RS14210 (HB365_14210)	-	2981148..2981840 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
HB365_RS14215 (HB365_14215)	pilT	2981967..2983004 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
HB365_RS14220 (HB365_14220)	pilU	2983032..2984150 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HB365_RS14225 (HB365_14225)	fur	2984220..2984657 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
HB365_RS14230 (HB365_14230)	-	2984769..2985167 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
HB365_RS14235 (HB365_14235)	-	2985222..2985545 (-)	324	WP_000442587.1	RnfH family protein	-
HB365_RS14240 (HB365_14240)	-	2985545..2986612 (-)	1068	WP_002019572.1	hypothetical protein	-
HB365_RS14245 (HB365_14245)	-	2986765..2987220 (-)	456	WP_000782976.1	bacteriohemerythrin	-
HB365_RS14250 (HB365_14250)	-	2987473..2988321 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=432365 HB365_RS14220 WP_000347039.1 2983032..2984150(+) (pilU) [Acinetobacter baumannii strain PM1912235]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=432365 HB365_RS14220 WP_000347039.1 2983032..2984150(+) (pilU) [Acinetobacter baumannii strain PM1912235]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395