Detailed information NTG0432338

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: HB365_RS01445
• Genome accession: NZ_CP050410
• Coordinates: 310199..311257 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain PM1912235
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 305199..316257

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HB365_RS01430 (HB365_01430)	-	306304..306636 (+)	333	WP_000169594.1	hypothetical protein	-
HB365_RS01435 (HB365_01435)	-	306639..307463 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
HB365_RS01440 (HB365_01440)	ponA	307481..310036 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
HB365_RS01445 (HB365_01445)	pilM	310199..311257 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
HB365_RS01450 (HB365_01450)	pilN	311257..311898 (+)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
HB365_RS01455 (HB365_01455)	pilO	311895..312635 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
HB365_RS01460 (HB365_01460)	pilP	312646..313173 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
HB365_RS01465 (HB365_01465)	pilQ	313241..315406 (+)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
HB365_RS01470 (HB365_01470)	aroK	315418..315987 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=432338 HB365_RS01445 WP_002014066.1 310199..311257(+) (pilM) [Acinetobacter baumannii strain PM1912235]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=432338 HB365_RS01445 WP_002014066.1 310199..311257(+) (pilM) [Acinetobacter baumannii strain PM1912235]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423