Detailed information NTG0432318

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HBN34_RS15465
• Genome accession: NZ_CP050403
• Coordinates: 3194201..3195319 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain VB2486
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3189201..3200319

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HBN34_RS15455 (HBN34_15455)	-	3192317..3193009 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
HBN34_RS15460 (HBN34_15460)	pilT	3193136..3194173 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
HBN34_RS15465 (HBN34_15465)	pilU	3194201..3195319 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HBN34_RS15470 (HBN34_15470)	fur	3195389..3195826 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
HBN34_RS15475 (HBN34_15475)	-	3195938..3196336 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
HBN34_RS15480 (HBN34_15480)	-	3196391..3196714 (-)	324	WP_000442585.1	RnfH family protein	-
HBN34_RS15485 (HBN34_15485)	-	3196714..3197781 (-)	1068	WP_000092403.1	hypothetical protein	-
HBN34_RS15490 (HBN34_15490)	-	3197934..3198389 (-)	456	WP_000782976.1	bacteriohemerythrin	-
HBN34_RS15495 (HBN34_15495)	-	3198642..3199495 (-)	854	Protein_3056	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=432318 HBN34_RS15465 WP_000347036.1 3194201..3195319(+) (pilU) [Acinetobacter baumannii strain VB2486]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=432318 HBN34_RS15465 WP_000347036.1 3194201..3195319(+) (pilU) [Acinetobacter baumannii strain VB2486]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392