Detailed information NTG0432147

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: HBK89_RS17240
• Genome accession: NZ_CP050388
• Coordinates: 3622286..3623344 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain VB473
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3617286..3628344

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HBK89_RS17215 (HBK89_17215)	aroK	3617556..3618125 (-)	570	WP_000643712.1	shikimate kinase AroK	-
HBK89_RS17220 (HBK89_17220)	pilQ	3618137..3620302 (-)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
HBK89_RS17225 (HBK89_17225)	pilP	3620370..3620897 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
HBK89_RS17230 (HBK89_17230)	pilO	3620908..3621648 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
HBK89_RS17235 (HBK89_17235)	pilN	3621645..3622286 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
HBK89_RS17240 (HBK89_17240)	pilM	3622286..3623344 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
HBK89_RS17245 (HBK89_17245)	ponA	3623507..3626062 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
HBK89_RS17250 (HBK89_17250)	-	3626080..3626904 (-)	825	WP_002019666.1	putative RNA methyltransferase	-
HBK89_RS17255 (HBK89_17255)	-	3626907..3627239 (-)	333	WP_000169594.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=432147 HBK89_RS17240 WP_002014066.1 3622286..3623344(-) (pilM) [Acinetobacter baumannii strain VB473]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=432147 HBK89_RS17240 WP_002014066.1 3622286..3623344(-) (pilM) [Acinetobacter baumannii strain VB473]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423