Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HB725_RS27730 Genome accession   NZ_CP050335
Coordinates   5970184..5971248 (-) Length   354 a.a.
NCBI ID   WP_126475841.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain DVT401     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5965184..5976248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB725_RS27705 (HB725_27760) aroK 5965719..5966237 (-) 519 WP_003095833.1 shikimate kinase AroK -
  HB725_RS27710 (HB725_27765) pilQ 5966249..5968393 (-) 2145 WP_174816580.1 type 4a pilus secretin PilQ Machinery gene
  HB725_RS27715 (HB725_27770) pilP 5968447..5968971 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  HB725_RS27720 (HB725_27775) pilO 5968968..5969591 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  HB725_RS27725 (HB725_27780) pilN 5969588..5970184 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  HB725_RS27730 (HB725_27785) pilM 5970184..5971248 (-) 1065 WP_126475841.1 type IV pilus assembly protein PilM Machinery gene
  HB725_RS27735 (HB725_27790) - 5971433..5973904 (+) 2472 WP_003125627.1 penicillin-binding protein 1A -
  HB725_RS27740 (HB725_27795) - 5974005..5975273 (-) 1269 WP_003103278.1 malic enzyme -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37967.44 Da        Isoelectric Point: 4.5405

>NTDB_id=431862 HB725_RS27730 WP_126475841.1 5970184..5971248(-) (pilM) [Pseudomonas aeruginosa strain DVT401]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGPSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=431862 HB725_RS27730 WP_126475841.1 5970184..5971248(-) (pilM) [Pseudomonas aeruginosa strain DVT401]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCCCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.899

100

0.562
  comM Acinetobacter nosocomialis M2

55.899

100

0.562
  comM Acinetobacter baylyi ADP1

51.977

100

0.52
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466