Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HBB04_RS15635 Genome accession   NZ_CP050260
Coordinates   3430302..3431366 (+) Length   354 a.a.
NCBI ID   WP_005888204.1    Uniprot ID   A0AA40P5K0
Organism   Pseudomonas coronafaciens strain X-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3425302..3436366
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBB04_RS15625 (HBB04_03156) - 3426219..3427487 (+) 1269 WP_005888200.1 malic enzyme-like NAD(P)-binding protein -
  HBB04_RS15630 (HBB04_03157) - 3427650..3430091 (-) 2442 WP_371903650.1 penicillin-binding protein 1A -
  HBB04_RS15635 (HBB04_03158) pilM 3430302..3431366 (+) 1065 WP_005888204.1 pilus assembly protein PilM Machinery gene
  HBB04_RS15640 (HBB04_03159) - 3431366..3431941 (+) 576 WP_005888206.1 PilN domain-containing protein -
  HBB04_RS15645 (HBB04_03160) pilO 3431938..3432561 (+) 624 WP_005888208.1 type 4a pilus biogenesis protein PilO -
  HBB04_RS15650 (HBB04_03161) pilP 3432558..3433085 (+) 528 WP_005888210.1 type 4a pilus biogenesis lipoprotein PilP -
  HBB04_RS15655 (HBB04_03162) pilQ 3433133..3435238 (+) 2106 WP_005888212.1 type IV pilus secretin PilQ Machinery gene
  HBB04_RS15660 (HBB04_03163) aroK 3435243..3435761 (+) 519 WP_005888214.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38103.67 Da        Isoelectric Point: 4.7688

>NTDB_id=430922 HBB04_RS15635 WP_005888204.1 3430302..3431366(+) (pilM) [Pseudomonas coronafaciens strain X-1]
MFELFKKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSAKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=430922 HBB04_RS15635 WP_005888204.1 3430302..3431366(+) (pilM) [Pseudomonas coronafaciens strain X-1]
GTGTTCGAACTCTTCAAGAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTGCCGGCCAACGCTGTGGTCGAAAAAAATA
TTGCCGAACTCGAAGGGGTGGGTCAGGCGCTGTCTCGCGTGCTTGCCAAGGCCAAGACCAGCGTCAAGATTGTGGCGGTG
GCCGTTGCCGGGTCCGCCGTTATTACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAAGCTGATCAGTACATTCCCTATCCGCTTGAAGAAGTCGCCATCGATTTTGAGGTGCAGGGCTATTCGGTCC
GCAACCCGGAACGCGTCGAGGTCCTTCTGGCGGCCTGCCGCAAGGAAAACGTCGAAGTGCGCGAGGCTGCGCTGGCGCTG
GCCGGTCTGACCGCGCGTGTCGTCGATGTCGAAGCCTATGCGCTGGAGCGTTCGTTCGGCCTGCTGGCCGCGCAACTGGG
CAACGGTCATGACGAGCTTACCGTGGCGGTGGTTGATATCGGCGCCACGATGACCACCTTGAGCGTGCTGCACCATGGCC
GGATCATCTATACCCGCGAACAACTGTTCGGTGGTCGCCAGCTGACTGACGAAATTCAGCGCCGTTACGGGCTCTCGATG
GAGGAAGCCGGGCTTGCCAAAAAGCAGGGCGGGCTGCCCGACGACTACGTCAGCGAAGTGCTGGACCCGTTCAAGGATGC
ATTGGTGCAGCAGGTTTCCCGTTCTCTGCAGTTCTTTTTTGCGGCAGGTCAGTACAACTCAGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCAATCTCCGGGCTGGAGCATCTTATTCAGCGGCGCATTGGTACGCCGACTATGGTGGCCAACCCG
TTTGCCGACATGGCTTTGAGCGCCAAGGTCAATGCTGGCGCCCTGGCGAGTGATGCGCCTGCCTTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

47.042

100

0.472