Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   HBA75_RS28435 Genome accession   NZ_CP050183
Coordinates   5571798..5572310 (-) Length   170 a.a.
NCBI ID   WP_000981966.1    Uniprot ID   A0A0A3WKS9
Organism   Bacillus thuringiensis strain HER1410     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 5542649..5577616 5571798..5572310 within 0


Gene organization within MGE regions


Location: 5542649..5577616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBA75_RS28280 (HBA75_28305) galE 5542649..5543665 (-) 1017 WP_000996540.1 UDP-glucose 4-epimerase GalE -
  HBA75_RS28285 (HBA75_28310) - 5543850..5544497 (-) 648 WP_000995363.1 hemolysin III family protein -
  HBA75_RS28290 (HBA75_28315) - 5544662..5545222 (+) 561 WP_000427879.1 DUF1836 domain-containing protein -
  HBA75_RS28295 (HBA75_28320) dbpA 5545263..5546708 (-) 1446 WP_000039350.1 ATP-dependent RNA helicase DbpA -
  HBA75_RS28300 (HBA75_28325) guaC 5547183..5548166 (+) 984 WP_000432447.1 GMP reductase -
  HBA75_RS28305 (HBA75_28330) pepF 5548302..5550119 (+) 1818 WP_086412008.1 oligoendopeptidase F -
  HBA75_RS28310 (HBA75_28335) - 5550181..5550417 (-) 237 Protein_5551 IS3 family transposase -
  HBA75_RS28315 (HBA75_28340) istB 5550471..5551223 (-) 753 WP_000798699.1 IS21-like element IS232 family helper ATPase IstB -
  HBA75_RS28320 (HBA75_28345) istA 5551213..5552508 (-) 1296 WP_000275580.1 IS21-like element IS232 family transposase -
  HBA75_RS28325 (HBA75_28350) - 5552588..5553250 (-) 663 WP_174402574.1 IS3 family transposase -
  HBA75_RS28330 (HBA75_28355) - 5553247..5553555 (-) 309 WP_086411305.1 helix-turn-helix domain-containing protein -
  HBA75_RS28335 (HBA75_28360) - 5553652..5554530 (-) 879 WP_000713633.1 radical SAM/SPASM domain-containing protein -
  HBA75_RS28340 (HBA75_28365) - 5554750..5556768 (-) 2019 WP_000253117.1 GAF domain-containing sensor histidine kinase -
  HBA75_RS28345 (HBA75_28370) rlmH 5556837..5557316 (-) 480 WP_001027000.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  HBA75_RS28350 (HBA75_28375) - 5557376..5557561 (-) 186 WP_001052827.1 CxxH/CxxC protein -
  HBA75_RS28355 (HBA75_28380) - 5557615..5558790 (-) 1176 WP_000008031.1 S1C family serine protease -
  HBA75_RS28360 (HBA75_28385) - 5558854..5559648 (-) 795 WP_000522837.1 MBL fold metallo-hydrolase -
  HBA75_RS28365 (HBA75_28390) - 5559632..5560474 (-) 843 WP_000383740.1 two-component system regulatory protein YycI -
  HBA75_RS28370 (HBA75_28395) yycH 5560455..5561771 (-) 1317 WP_000061560.1 two-component system activity regulator YycH -
  HBA75_RS28375 (HBA75_28400) walK 5561768..5563609 (-) 1842 WP_000755367.1 cell wall metabolism sensor histidine kinase WalK -
  HBA75_RS28380 (HBA75_28405) walR 5563613..5564320 (-) 708 WP_000971865.1 cell wall metabolism DNA-binding response regulator WalR -
  HBA75_RS28405 (HBA75_28430) - 5565115..5566404 (-) 1290 WP_000100238.1 adenylosuccinate synthase -
  HBA75_RS28410 (HBA75_28435) dnaB 5566620..5567981 (-) 1362 WP_001286148.1 replicative DNA helicase -
  HBA75_RS28415 (HBA75_28440) rplI 5568009..5568455 (-) 447 WP_000864228.1 50S ribosomal protein L9 -
  HBA75_RS28420 (HBA75_28445) - 5568452..5570425 (-) 1974 WP_001113785.1 DHH family phosphoesterase -
  HBA75_RS28425 (HBA75_28450) - 5570504..5571439 (-) 936 WP_000814837.1 YybS family protein -
  HBA75_RS28430 (HBA75_28455) rpsR 5571519..5571752 (-) 234 WP_000918873.1 30S ribosomal protein S18 -
  HBA75_RS28435 (HBA75_28460) ssbA 5571798..5572310 (-) 513 WP_000981966.1 single-stranded DNA-binding protein Machinery gene
  HBA75_RS28440 (HBA75_28465) rpsF 5572337..5572627 (-) 291 WP_001233778.1 30S ribosomal protein S6 -
  HBA75_RS28445 (HBA75_28470) ychF 5572819..5573919 (-) 1101 WP_000524657.1 redox-regulated ATPase YchF -
  HBA75_RS28450 (HBA75_28475) - 5574035..5574232 (-) 198 WP_000435485.1 DUF951 domain-containing protein -
  HBA75_RS28455 (HBA75_28480) - 5574253..5575134 (-) 882 WP_000364814.1 mechanosensitive ion channel family protein -
  HBA75_RS28460 (HBA75_28485) yyaC 5575394..5575990 (+) 597 WP_001020714.1 spore protease YyaC -
  HBA75_RS28465 (HBA75_28490) spo0J 5576011..5576862 (-) 852 WP_001051832.1 stage 0 sporulation protein Spo0J -
  HBA75_RS28470 (HBA75_28495) soj 5576855..5577616 (-) 762 WP_000516115.1 sporulation initiation inhibitor protein Soj -

Sequence


Protein


Download         Length: 170 a.a.        Molecular weight: 18494.29 Da        Isoelectric Point: 4.9327

>NTDB_id=430651 HBA75_RS28435 WP_000981966.1 5571798..5572310(-) (ssbA) [Bacillus thuringiensis strain HER1410]
MMNRVILVGRLTKDPDLRYTPNGVAVATFTLAVNRAFANQQGEREADFINCVIWRKQAENVANYLKKGSLAGVDGRLQTR
NYEGQDGKRVYVTEVLAESVQFLEPRNGGGEQRGSFNQQPSGAGFGNQGSNPFGQSSNSGNQGNSGFTKNDDPFSNVGQP
IDISDDDLPF

Nucleotide


Download         Length: 513 bp        

>NTDB_id=430651 HBA75_RS28435 WP_000981966.1 5571798..5572310(-) (ssbA) [Bacillus thuringiensis strain HER1410]
TTGATGAATCGTGTTATCCTCGTTGGTCGTTTAACTAAGGACCCTGACTTACGTTACACGCCCAATGGTGTCGCAGTAGC
TACTTTTACGTTAGCTGTGAATCGCGCATTTGCCAATCAGCAAGGTGAGCGTGAAGCTGACTTTATTAATTGTGTAATAT
GGCGTAAACAAGCAGAAAACGTAGCAAATTATTTGAAAAAAGGTAGCTTAGCAGGCGTAGATGGACGTCTTCAAACTCGT
AATTACGAGGGACAAGATGGTAAACGTGTATACGTAACAGAAGTTCTTGCGGAAAGCGTGCAATTTTTAGAGCCACGTAA
TGGCGGTGGGGAGCAACGTGGTTCATTTAATCAGCAACCATCAGGAGCTGGTTTCGGTAACCAAGGCTCTAACCCATTTG
GTCAATCTAGTAATTCAGGTAACCAAGGTAATTCTGGATTTACGAAGAATGACGATCCATTTTCAAATGTAGGTCAACCG
ATCGACATTTCCGACGACGATTTACCGTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0A3WKS9

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

72.571

100

0.747

  ssb Latilactobacillus sakei subsp. sakei 23K

58.621

100

0.6

  ssbB Bacillus subtilis subsp. subtilis str. 168

65.094

62.353

0.406

  ssb Glaesserella parasuis strain SC1401

37.43

100

0.394