Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   G7079_RS12195 Genome accession   NZ_CP049872
Coordinates   2569598..2570461 (+) Length   287 a.a.
NCBI ID   WP_166057566.1    Uniprot ID   A0A6G7ZXI3
Organism   Thermomonas sp. HDW16     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2564598..2575461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7079_RS12180 (G7079_12185) - 2565591..2566460 (-) 870 WP_166057563.1 glycosyltransferase family 2 protein -
  G7079_RS12185 (G7079_12190) pilB 2566590..2568314 (+) 1725 WP_166057564.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G7079_RS12190 (G7079_12195) pilC 2568326..2569597 (+) 1272 WP_166057565.1 type II secretion system F family protein Machinery gene
  G7079_RS12195 (G7079_12200) pilD 2569598..2570461 (+) 864 WP_166057566.1 A24 family peptidase Machinery gene
  G7079_RS12200 (G7079_12205) coaE 2570498..2571109 (+) 612 WP_166057567.1 dephospho-CoA kinase -
  G7079_RS12205 (G7079_12210) - 2571085..2572059 (-) 975 WP_166057568.1 Nudix family hydrolase -
  G7079_RS12210 (G7079_12215) secA 2572118..2574838 (-) 2721 WP_166057569.1 preprotein translocase subunit SecA -

Sequence

Protein


Download         Length: 287 a.a.        Molecular weight: 31403.12 Da        Isoelectric Point: 8.3297

>NTDB_id=428312 G7079_RS12195 WP_166057566.1 2569598..2570461(+) (pilD) [Thermomonas sp. HDW16]
MAYLDAHPGLGYPAAAGLGLLVGSFLNVVILRLPRRLEWQWKRDAREILEEPELYDPPPPGIVVERSHCPHCGHELSWYE
NIPVFSWLALRGKCRSCKAPISIQYPLVELLTAALFLACVSRFGFGWQGFGAMLLTGFLIAMSGIDLRTRLLPDQLTLPL
MWLGFVASIENLYVGQKAALLGAMAGYLSLWSVYWLFKQLTGKEGMGHGDFKLLAALGAWTGLAGVLPTVLISSLVGAVI
GSIWLAVKGKDKATPIPFGPYLAIAGWITFFWGHDLVGAYMRFAGLA

Nucleotide


Download         Length: 864 bp        

>NTDB_id=428312 G7079_RS12195 WP_166057566.1 2569598..2570461(+) (pilD) [Thermomonas sp. HDW16]
ATGGCCTATCTGGATGCACACCCCGGCCTCGGCTATCCCGCCGCGGCCGGCTTGGGGTTGCTGGTCGGCAGCTTCCTGAA
CGTGGTGATCCTGCGGCTGCCGCGTCGGCTTGAGTGGCAGTGGAAGCGCGACGCGCGCGAAATACTGGAAGAACCCGAGC
TCTACGACCCACCGCCACCGGGGATCGTGGTGGAACGCTCGCATTGTCCGCATTGCGGGCACGAACTGTCCTGGTACGAG
AACATCCCCGTCTTCAGCTGGCTGGCGCTGCGCGGCAAGTGCCGCAGTTGCAAGGCACCGATTTCGATCCAGTATCCGCT
GGTCGAACTGCTGACGGCAGCACTGTTCCTCGCCTGCGTGTCGCGCTTCGGCTTCGGTTGGCAGGGCTTTGGCGCGATGC
TGCTCACCGGCTTCCTGATCGCGATGTCCGGCATCGACCTGCGCACGCGATTGTTGCCGGATCAACTCACGTTGCCCTTG
ATGTGGCTGGGTTTCGTCGCCTCGATCGAGAACCTGTACGTTGGCCAGAAAGCCGCCCTGCTGGGCGCGATGGCCGGCTA
CTTGAGCCTGTGGTCGGTGTACTGGCTGTTCAAGCAGCTCACCGGCAAGGAAGGCATGGGCCATGGCGACTTCAAATTGC
TGGCGGCGCTCGGCGCGTGGACGGGCCTGGCCGGGGTGCTGCCGACGGTGCTGATCTCGTCGCTGGTCGGTGCGGTGATC
GGCTCGATCTGGCTGGCGGTCAAGGGCAAGGACAAGGCGACGCCCATTCCATTCGGCCCTTACCTCGCGATTGCCGGCTG
GATCACCTTCTTTTGGGGTCATGACCTGGTCGGCGCATATATGCGTTTCGCCGGCCTGGCTTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-123)

Type IV leader peptidase family (Peptidase_A24)

(133-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G7ZXI3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

49.47

98.606

0.488
  pilD Vibrio cholerae strain A1552

50.909

95.819

0.488
  pilD Acinetobacter baumannii D1279779

46.528

100

0.467
  pilD Acinetobacter nosocomialis M2

46.454

98.258

0.456
  pilD Neisseria gonorrhoeae MS11

45.387

94.425

0.429