Detailed information NTG0428238

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: G7069_RS01750
• Genome accession: NZ_CP049864
• Coordinates: 361260..362447 (+)
• Length: 395 a.a.
• NCBI ID: WP_166293664.1
• Uniprot ID: -
• Organism: Lysobacter sp. HDW10
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 356260..367447

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G7069_RS01730 (G7069_01730)	-	357130..357948 (-)	819	WP_166293657.1	ABC transporter permease	-
G7069_RS01735 (G7069_01735)	-	358005..359186 (-)	1182	WP_166293660.1	cystathionine gamma-synthase	-
G7069_RS01740 (G7069_01740)	-	359277..360653 (-)	1377	WP_166293662.1	pyridoxal-phosphate dependent enzyme	-
G7069_RS01745 (G7069_01745)	-	360823..361041 (-)	219	WP_166297422.1	YdcH family protein	-
G7069_RS01750 (G7069_01750)	pilU	361260..362447 (+)	1188	WP_166293664.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
G7069_RS01755 (G7069_01755)	maiA	362460..363128 (-)	669	WP_166293666.1	maleylacetoacetate isomerase	-
G7069_RS01760 (G7069_01760)	-	363125..364108 (-)	984	WP_166293668.1	fumarylacetoacetate hydrolase family protein	-
G7069_RS01765 (G7069_01765)	-	364175..364924 (-)	750	WP_166293670.1	2OG-Fe dioxygenase family protein	-
G7069_RS01770 (G7069_01770)	rpsI	365004..365396 (-)	393	WP_166293672.1	30S ribosomal protein S9	-
G7069_RS01775 (G7069_01775)	rplM	365409..365837 (-)	429	WP_166293675.1	50S ribosomal protein L13	-
G7069_RS01780 (G7069_01780)	coq7	366006..366674 (+)	669	WP_166293677.1	2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase	-

Sequence

Protein

• Length: 395 a.a.
• Molecular weight: 43804.07 Da
• Isoelectric Point: 6.2293

>NTDB_id=428238 G7069_RS01750 WP_166293664.1 361260..362447(+) (pilU) [Lysobacter sp. HDW10]
MDIGHFLKLMMQKRGSDMFLTTGAPIHVKIEGKMHPLGTTALPPGLVRRIANSLMDEDQRATFERELELNMGLMVEDIGR
FRVNVFKQRGEVGMVIRAIRTEIPTINELQLPDTLKTLISAPRGLILVVGSTGSGKSTTLASMIDHRNSHMSGHILTIED
PIEYLHRHKRSLVNQREVGIDTHGFHNALKNAMRETPDVILIGEILDAHTMESAIAFAETGHLCLATLHANNADQAIERI
LNFFPESAHKNVLMNLSLNLHAVVALRLISGSDGKRLPASEVLINTQLIRDLMRRGQVHAIKQAMEESLQDGMHTFDQSL
FRLTKAGRISEEDALRAADSRDGLAVKFRLSDGAGEEHDAYAEIFATQAADAIKEDDDAGIPKATEFSNNFEEFQ

Nucleotide

• Length: 1188 bp

>NTDB_id=428238 G7069_RS01750 WP_166293664.1 361260..362447(+) (pilU) [Lysobacter sp. HDW10]
ATGGATATCGGTCACTTTCTGAAACTCATGATGCAAAAGCGCGGGTCGGACATGTTCCTGACCACCGGTGCGCCGATTCA
CGTCAAGATTGAAGGCAAGATGCATCCCTTGGGCACCACGGCATTGCCGCCGGGCTTGGTGCGCAGAATCGCCAATTCGT
TGATGGATGAAGATCAACGCGCCACGTTCGAACGTGAGTTGGAACTCAACATGGGCTTGATGGTCGAAGACATCGGCCGC
TTCCGTGTCAACGTGTTTAAGCAACGCGGCGAAGTGGGCATGGTGATTCGTGCGATTCGCACAGAGATCCCAACGATCAA
CGAACTGCAATTGCCCGACACACTGAAAACGTTGATTTCCGCACCACGCGGTTTGATTTTGGTGGTGGGTTCAACCGGTT
CGGGTAAGTCCACCACCTTGGCTTCGATGATCGATCATCGCAATAGCCATATGAGTGGCCACATTTTGACGATCGAAGAT
CCGATCGAATACTTGCATCGCCACAAACGTTCTTTGGTGAACCAGCGCGAAGTCGGGATTGATACGCATGGGTTCCATAA
CGCGCTGAAGAACGCGATGCGTGAAACACCCGATGTCATCTTGATCGGTGAAATCTTGGATGCACACACGATGGAATCCG
CCATTGCGTTCGCAGAAACCGGGCATTTGTGCCTGGCGACATTGCACGCAAACAACGCTGACCAAGCCATCGAACGCATT
CTGAACTTCTTCCCGGAATCAGCGCATAAGAACGTGCTGATGAATCTTTCGCTGAACTTGCACGCTGTGGTCGCACTTCG
ATTGATTAGCGGTAGTGACGGCAAGCGTTTGCCGGCGTCGGAAGTGTTGATCAATACGCAGTTGATTCGCGACTTGATGC
GTCGGGGTCAAGTGCATGCCATCAAACAAGCGATGGAAGAATCACTTCAAGACGGCATGCATACCTTCGATCAGTCTTTG
TTCCGACTCACGAAGGCGGGTCGTATCAGCGAGGAAGATGCGCTGCGTGCCGCCGATTCGAGAGATGGTCTGGCCGTGAA
GTTCCGTTTGTCCGATGGTGCGGGCGAAGAGCACGATGCCTACGCAGAAATCTTTGCGACCCAAGCCGCGGATGCCATCA
AAGAAGACGACGACGCAGGTATTCCGAAGGCGACGGAATTCAGCAACAACTTCGAAGAATTTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-268)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	50.394	96.456	0.486
pilU	Vibrio cholerae strain A1552	49.153	89.62	0.441
pilU	Acinetobacter baylyi ADP1	48.199	91.392	0.441
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.895	87.089	0.382