Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MEALZ_RS15815 Genome accession   NC_016112
Coordinates   3754658..3755719 (-) Length   353 a.a.
NCBI ID   WP_014149663.1    Uniprot ID   -
Organism   Methylotuvimicrobium alcaliphilum 20Z     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3749658..3760719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MEALZ_RS15790 (MEALZ_3235) aroK 3749873..3750391 (-) 519 WP_046061206.1 shikimate kinase AroK -
  MEALZ_RS15795 (MEALZ_3236) pilQ 3750466..3752826 (-) 2361 WP_223842319.1 type IV pilus secretin family protein -
  MEALZ_RS15800 (MEALZ_3237) - 3752867..3753463 (-) 597 WP_014149660.1 pilus assembly protein PilP -
  MEALZ_RS15805 (MEALZ_3238) - 3753460..3754095 (-) 636 WP_014149661.1 type 4a pilus biogenesis protein PilO -
  MEALZ_RS15810 (MEALZ_3239) - 3754095..3754658 (-) 564 WP_014149662.1 PilN domain-containing protein -
  MEALZ_RS15815 (MEALZ_3240) pilM 3754658..3755719 (-) 1062 WP_014149663.1 pilus assembly protein PilM Machinery gene
  MEALZ_RS15820 (MEALZ_3241) - 3756719..3759085 (+) 2367 WP_014149664.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38459.94 Da        Isoelectric Point: 4.6647

>NTDB_id=42723 MEALZ_RS15815 WP_014149663.1 3754658..3755719(-) (pilM) [Methylotuvimicrobium alcaliphilum 20Z]
MNWFSRKNVPVVGIDISTAAVKLLELSKNGPRYRVESYAVAPLPQDAVIDKNFANVDVISDAIKQAIKQSGTKVKYACVA
VAGSAVMTKIITMPANYSDEEVYEQIMVEADQYVPYALDEVNLDFEVQGKNAKNSEMVDVLLVASRRENVEDRIEALDRA
GLKAKIIDVEAFAMENAFALIADQLPGSSEGQTIAISDIGSTMATLNVIADGRTIYTREQGFGGRQLTDEIQRRYGLSYE
EAGLAKRHGGLPDNYINDVLEPFKKAMVQQIARSLQFFVSSSANRTIDSIILAGGCSSIPGVEKLVEQNLSIPCFIANPF
INMALSNRVKPQSLSSDAPAMMIACGLALRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=42723 MEALZ_RS15815 WP_014149663.1 3754658..3755719(-) (pilM) [Methylotuvimicrobium alcaliphilum 20Z]
ATGAACTGGTTTAGTCGCAAAAATGTGCCTGTCGTCGGTATCGACATTAGCACGGCAGCCGTTAAGTTATTGGAATTATC
GAAAAACGGTCCTCGTTATCGAGTCGAAAGTTATGCCGTCGCGCCATTACCGCAGGATGCCGTTATCGATAAAAATTTCG
CGAATGTCGATGTAATCTCTGACGCCATCAAACAAGCTATCAAACAATCCGGAACGAAAGTCAAATATGCCTGCGTCGCG
GTTGCGGGTTCGGCGGTTATGACCAAAATCATAACGATGCCTGCTAATTATTCGGACGAAGAAGTTTACGAACAAATCAT
GGTCGAGGCCGATCAATATGTGCCTTATGCACTGGATGAGGTCAACCTGGATTTTGAAGTTCAGGGAAAAAACGCTAAAA
ACTCCGAAATGGTTGATGTGCTCTTGGTCGCCTCCCGTCGTGAAAATGTCGAGGACCGCATCGAAGCCTTGGATAGGGCA
GGTTTAAAAGCCAAGATCATCGATGTCGAAGCGTTTGCGATGGAAAACGCTTTTGCGCTTATCGCCGATCAATTACCCGG
TTCTAGCGAAGGTCAGACTATCGCTATCTCGGATATCGGTTCGACAATGGCGACTTTGAACGTCATTGCGGACGGTAGAA
CCATTTATACGCGCGAACAAGGCTTCGGCGGAAGGCAGTTGACCGATGAAATTCAACGCCGCTACGGTTTGTCCTACGAA
GAAGCCGGTCTTGCCAAGCGGCATGGCGGTCTTCCCGATAATTATATCAATGACGTATTGGAGCCATTTAAGAAGGCGAT
GGTTCAGCAAATCGCCCGATCGCTCCAATTTTTTGTTTCATCGAGCGCCAATAGAACGATCGATTCGATTATTTTGGCGG
GCGGTTGCTCGTCGATACCCGGTGTCGAAAAGCTGGTCGAGCAGAACTTGAGTATTCCCTGTTTTATTGCCAATCCGTTT
ATCAATATGGCTTTATCGAATCGAGTGAAACCGCAGAGTTTAAGTAGCGATGCGCCGGCGATGATGATTGCCTGCGGTCT
TGCGCTAAGGAGTTTCGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.708

100

0.507
  comM Acinetobacter nosocomialis M2

50.708

100

0.507
  comM Acinetobacter baylyi ADP1

48.442

100

0.484
  pilM Legionella pneumophila strain ERS1305867

41.36

100

0.414

Multiple sequence alignment