Detailed information NTG0042710

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MEALZ_RS02245
• Genome accession: NC_016112
• Coordinates: 497256..498431 (-)
• Length: 391 a.a.
• NCBI ID: WP_014146965.1
• Uniprot ID: -
• Organism: Methylotuvimicrobium alcaliphilum 20Z
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 492256..503431

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MEALZ_RS02235 (MEALZ_0455)	-	493067..495406 (-)	2340	WP_014146963.1	alpha/beta fold hydrolase	-
MEALZ_RS02240 (MEALZ_0456)	-	495915..496406 (+)	492	WP_084685258.1	hypothetical protein	-
MEALZ_RS02245 (MEALZ_0457)	pilU	497256..498431 (-)	1176	WP_014146965.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MEALZ_RS02250 (MEALZ_0458)	-	498945..499988 (+)	1044	WP_014146966.1	tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase	-
MEALZ_RS02255 (MEALZ_0459)	-	499985..500212 (+)	228	WP_014146967.1	sulfurtransferase TusA family protein	-
MEALZ_RS02260 (MEALZ_0460)	-	500212..501354 (+)	1143	WP_014146968.1	cation diffusion facilitator family transporter	-
MEALZ_RS02265 (MEALZ_0461)	argJ	501818..503032 (+)	1215	WP_014146969.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 391 a.a.
• Molecular weight: 43452.60 Da
• Isoelectric Point: 5.4953

>NTDB_id=42710 MEALZ_RS02245 WP_014146965.1 497256..498431(-) (pilU) [Methylotuvimicrobium alcaliphilum 20Z]
MDFKDYLKILVNKDGSDLYLTVGAPAAAKFQGALKPLESEKLTGERIKAIAYSLMDADQQKDFEHVPEMNLAITEQGIGR
FRVNIFKQRNNLALVIRNIKVDIPNADQLGLPEVLKKVIMEKRGLILFVGGTGSGKSTSLAALIDHRNSNSSGHIITIED
PIEYLHPHKRCLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRNQLLLDLSLNLKAFVSQRLVPTVDGKRVAAIEILLGTQLVKDLIHKGEIHTIKEAMEKSENIGMQTFDSHL
LKLFKEGKISLEEALQNSDSPNNLKLKINLSEGKGSATEAVAKSTGGLTSRFSLEAIEKDQEEEEEEEEQS

Nucleotide

• Length: 1176 bp

>NTDB_id=42710 MEALZ_RS02245 WP_014146965.1 497256..498431(-) (pilU) [Methylotuvimicrobium alcaliphilum 20Z]
ATGGATTTTAAAGATTATCTAAAAATTTTGGTCAACAAAGACGGTTCCGATCTTTACTTAACGGTCGGCGCGCCGGCCGC
GGCAAAATTTCAAGGCGCATTGAAACCGCTCGAAAGCGAAAAACTGACCGGCGAACGCATCAAGGCTATCGCTTACAGTT
TAATGGATGCCGATCAGCAAAAAGACTTCGAACACGTCCCGGAAATGAATCTGGCGATCACCGAACAGGGCATCGGCCGA
TTCCGGGTCAATATTTTCAAACAGCGCAACAACCTGGCCTTGGTCATTCGAAACATCAAGGTCGATATTCCGAATGCCGA
TCAATTAGGCCTGCCCGAAGTGCTGAAAAAAGTCATCATGGAAAAGCGCGGTTTGATTCTATTCGTCGGCGGCACCGGCT
CCGGCAAGTCGACCTCCCTGGCCGCACTGATCGATCACCGCAACAGCAATTCGTCGGGGCACATCATCACGATCGAAGAC
CCGATCGAATATCTTCATCCGCACAAACGCTGCCTGGTCAATCAACGCGAAGTCGGCGTCGATACGCTCAGTTACGAGGA
CGCGTTGAAAAATACGCTACGCCAGGCACCGGACGTGATTCTGATCGGCGAAATCCGCTCTCAAGAAACGATGGAACATG
CACTCGCCTTCGCCGAAACCGGGCATTTATGCTTGTCGACGCTGCACGCCAACAACGCCAATCAGGCCCTGGACCGTATC
ATCAATTTCTTCCCGGAAGAGCGGCGTAACCAATTATTGCTGGATCTATCGCTGAATTTGAAGGCCTTTGTTTCGCAAAG
ACTTGTTCCTACCGTGGACGGAAAACGGGTCGCGGCGATCGAAATTTTGCTGGGGACGCAATTGGTCAAAGACTTGATTC
ATAAAGGCGAAATTCATACGATCAAGGAAGCGATGGAAAAATCCGAAAACATCGGCATGCAAACCTTCGACAGCCATTTG
CTCAAACTTTTCAAGGAAGGCAAAATTTCACTCGAAGAAGCCTTGCAAAACTCCGACTCGCCGAATAACTTGAAATTGAA
AATCAACCTCAGCGAAGGCAAGGGATCGGCGACCGAAGCGGTCGCTAAATCGACTGGCGGCTTGACTTCAAGATTCTCGC
TCGAGGCGATTGAAAAGGATCAGGAGGAAGAAGAAGAAGAAGAAGAGCAAAGCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (69-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56	95.908	0.537
pilU	Acinetobacter baylyi ADP1	53.955	90.537	0.488
pilU	Vibrio cholerae strain A1552	50.847	90.537	0.46
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.667	92.072	0.384