Detailed information NTG0425677

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MFABIIPG_RS13625
• Genome accession: NZ_CP049240
• Coordinates: 2896647..2897765 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain Ax270
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2891647..2902765

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MFABIIPG_RS13615 (MFABIIPG_02716)	-	2894763..2895455 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
MFABIIPG_RS13620 (MFABIIPG_02717)	pilT	2895582..2896619 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
MFABIIPG_RS13625 (MFABIIPG_02718)	pilU	2896647..2897765 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MFABIIPG_RS13630 (MFABIIPG_02719)	fur	2897835..2898272 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
MFABIIPG_RS13635 (MFABIIPG_02720)	-	2898384..2898782 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
MFABIIPG_RS13640 (MFABIIPG_02721)	-	2898837..2899160 (-)	324	WP_000442585.1	RnfH family protein	-
MFABIIPG_RS13645 (MFABIIPG_02722)	-	2899160..2900227 (-)	1068	WP_000092403.1	hypothetical protein	-
MFABIIPG_RS13650 (MFABIIPG_02723)	-	2900380..2900835 (-)	456	WP_000782976.1	bacteriohemerythrin	-
MFABIIPG_RS13655 (MFABIIPG_02724)	-	2901088..2901936 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=425677 MFABIIPG_RS13625 WP_000347036.1 2896647..2897765(+) (pilU) [Acinetobacter baumannii strain Ax270]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=425677 MFABIIPG_RS13625 WP_000347036.1 2896647..2897765(+) (pilU) [Acinetobacter baumannii strain Ax270]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392