Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   ST64987_RS08880 Genome accession   NZ_CP049053
Coordinates   1684595..1685032 (-) Length   145 a.a.
NCBI ID   WP_011681686.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain ST64987     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1679595..1690032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ST64987_RS08840 (ST64987_1691) - 1679659..1680321 (-) 663 WP_011681679.1 CPBP family intramembrane glutamic endopeptidase -
  ST64987_RS08845 (ST64987_1692) - 1680418..1680861 (-) 444 WP_011681680.1 hypothetical protein -
  ST64987_RS10400 (ST64987_1693) - 1680935..1681360 (-) 426 WP_014608739.1 CPBP family intramembrane glutamic endopeptidase -
  ST64987_RS10405 (ST64987_1694) - 1681317..1681592 (-) 276 WP_011681681.1 hypothetical protein -
  ST64987_RS08860 (ST64987_1695) - 1681604..1681801 (-) 198 WP_011681682.1 helix-turn-helix transcriptional regulator -
  ST64987_RS08865 (ST64987_1696) - 1682050..1683243 (-) 1194 WP_014608740.1 acetate kinase -
  ST64987_RS08870 (ST64987_1697) comYH 1683299..1684255 (-) 957 WP_011681684.1 class I SAM-dependent methyltransferase Machinery gene
  ST64987_RS08875 (ST64987_1698) comGG 1684300..1684617 (-) 318 WP_011681685.1 competence type IV pilus minor pilin ComGG -
  ST64987_RS08880 (ST64987_1699) comYF 1684595..1685032 (-) 438 WP_011681686.1 competence type IV pilus minor pilin ComGF Machinery gene
  ST64987_RS08885 (ST64987_1700) comGE 1685016..1685309 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  ST64987_RS08890 (ST64987_1701) comYD 1685281..1685709 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  ST64987_RS08895 (ST64987_1702) comYC 1685669..1685995 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  ST64987_RS08900 (ST64987_1703) comYB 1685992..1687092 (-) 1101 WP_120764773.1 competence type IV pilus assembly protein ComGB Machinery gene
  ST64987_RS08905 (ST64987_1704) comGA/cglA/cilD 1686974..1687915 (-) 942 WP_014608745.1 competence type IV pilus ATPase ComGA Machinery gene
  ST64987_RS08910 (ST64987_1705) - 1687996..1688358 (-) 363 WP_167401603.1 DUF1033 family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16375.85 Da        Isoelectric Point: 8.4500

>NTDB_id=424500 ST64987_RS08880 WP_011681686.1 1684595..1685032(-) (comYF) [Streptococcus thermophilus strain ST64987]
MYLSSKLRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVRQQENQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSASDFRKTNADGRGYQPMLFDVKSSKVSQKNQIVTIQVTLKNELQRSFVYAFEKTG

Nucleotide


Download         Length: 438 bp        

>NTDB_id=424500 ST64987_RS08880 WP_011681686.1 1684595..1685032(-) (comYF) [Streptococcus thermophilus strain ST64987]
ATGTATCTAAGCAGTAAGCTGCGTGCCTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTCATTGCTGGCTCAGTTCA
AGTTTATCAAGGGTTAACCACTGTACTTGTGAGTAATGTTAAGCAGGTAAGACAACAAGAAAACCAAGATTGGCTTCTAT
TTGTTCAACAAATGGAGGCAGAATTAGAAGGATGTCAGTTGGTCAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTGGCTTTTGGTCTTTCAAGTGCCTCTGATTTTCGAAAAACAAATGCTGATGGTCGAGGTTATCAACCAAT
GCTTTTCGATGTCAAATCTAGTAAGGTAAGTCAAAAGAACCAGATAGTTACCATCCAAGTGACTCTTAAAAATGAACTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(39-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

58.156

97.241

0.566
  comYF Streptococcus mutans UA159

57.447

97.241

0.559
  comGF/cglF Streptococcus pneumoniae D39

49.286

96.552

0.476
  comGF/cglF Streptococcus pneumoniae Rx1

49.286

96.552

0.476
  comGF/cglF Streptococcus pneumoniae R6

49.286

96.552

0.476
  comGF/cglF Streptococcus pneumoniae TIGR4

49.286

96.552

0.476
  comGF/cglF Streptococcus mitis NCTC 12261

47.518

97.241

0.462
  comGF/cglF Streptococcus mitis SK321

46.809

97.241

0.455
  comGF Lactococcus lactis subsp. cremoris KW2

45.324

95.862

0.434