Detailed information NTG0423496

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AbD4_RS04960
• Genome accession: NZ_CP048849
• Coordinates: 1026406..1027524 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain D4
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1021406..1032524

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AbD4_RS04930 (AbD4_00990)	-	1022236..1023084 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
AbD4_RS04935 (AbD4_00991)	-	1023336..1023791 (+)	456	WP_000782976.1	bacteriohemerythrin	-
AbD4_RS04940 (AbD4_00992)	-	1023944..1025011 (+)	1068	WP_004736014.1	hypothetical protein	-
AbD4_RS04945 (AbD4_00993)	-	1025011..1025334 (+)	324	WP_000442582.1	RnfH family protein	-
AbD4_RS04950 (AbD4_00994)	-	1025389..1025787 (-)	399	WP_001170993.1	outer membrane protein assembly factor BamE	-
AbD4_RS04955 (AbD4_00995)	fur	1025899..1026336 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
AbD4_RS04960 (AbD4_00996)	pilU	1026406..1027524 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AbD4_RS04965 (AbD4_00997)	pilT	1027552..1028589 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
AbD4_RS04970 (AbD4_00998)	-	1028716..1029408 (+)	693	WP_001108513.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=423496 AbD4_RS04960 WP_000347039.1 1026406..1027524(-) (pilU) [Acinetobacter baumannii strain D4]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=423496 AbD4_RS04960 WP_000347039.1 1026406..1027524(-) (pilU) [Acinetobacter baumannii strain D4]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCGCTTAAACATGCTGATTCACCGAACGATTTGCG
TTTAACCATTAAACTTGCAGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395