Detailed information NTG0422907

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IFJ77_RS14135
• Genome accession: NZ_CP061780
• Coordinates: 3043339..3044487 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain ZBX-P11
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3038339..3049487

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IFJ77_RS14110 (IFJ77_14110)	-	3039490..3039786 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
IFJ77_RS14115 (IFJ77_14115)	-	3039783..3040376 (-)	594	WP_003084544.1	YggT family protein	-
IFJ77_RS14120 (IFJ77_14120)	proC	3040387..3041208 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
IFJ77_RS14125 (IFJ77_14125)	-	3041220..3041912 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
IFJ77_RS14130 (IFJ77_14130)	pilT	3042127..3043161 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
IFJ77_RS14135 (IFJ77_14135)	pilU	3043339..3044487 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
IFJ77_RS14140 (IFJ77_14140)	czcD	3044495..3045394 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
IFJ77_RS14145 (IFJ77_14145)	-	3045549..3045953 (+)	405	WP_003084560.1	NINE protein	-
IFJ77_RS14150 (IFJ77_14150)	-	3046196..3047569 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
IFJ77_RS14155 (IFJ77_14155)	-	3047566..3048750 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=422907 IFJ77_RS14135 WP_003110016.1 3043339..3044487(+) (pilU) [Pseudomonas aeruginosa strain ZBX-P11]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=422907 IFJ77_RS14135 WP_003110016.1 3043339..3044487(+) (pilU) [Pseudomonas aeruginosa strain ZBX-P11]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCTGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382