Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   WFL_RS10430 Genome accession   NC_017664
Coordinates   2090338..2091348 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli W     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2085338..2096348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WFL_RS10410 (WFL_09970) znuA 2086363..2087295 (-) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  WFL_RS10415 (WFL_09975) znuC 2087374..2088129 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  WFL_RS10420 (WFL_09980) znuB 2088126..2088911 (+) 786 WP_000571465.1 zinc ABC transporter permease subunit ZnuB -
  WFL_RS10425 (WFL_09985) - 2089090..2090070 (-) 981 WP_000399648.1 IS110-like element IS621 family transposase -
  WFL_RS10430 (WFL_09990) ruvB 2090338..2091348 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  WFL_RS10435 (WFL_09995) ruvA 2091357..2091968 (-) 612 WP_000580328.1 Holliday junction branch migration protein RuvA -
  WFL_RS26935 yobI 2092107..2092172 (-) 66 WP_001386853.1 stress response small protein YobI -
  WFL_RS10440 (WFL_10000) yebB 2092243..2092845 (+) 603 WP_001024917.1 YebB family permuted papain-like enzyme -
  WFL_RS10445 (WFL_10005) ruvC 2092847..2093368 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  WFL_RS10450 (WFL_10010) yebC 2093403..2094143 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  WFL_RS10455 (WFL_10015) nudB 2094172..2094624 (-) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=42282 WFL_RS10430 WP_000568519.1 2090338..2091348(-) (ruvB) [Escherichia coli W]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=42282 WFL_RS10430 WP_000568519.1 2090338..2091348(-) (ruvB) [Escherichia coli W]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACACGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGCTGAATGTCGATGCTGAAGGTTTTGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAATTCTTTGGTGGGCCTGTAGGTCTGGATAACCTGGCGGCGGCTATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAGCCTTATCTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment