Detailed information NTG0422689

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IFJ79_RS10195
• Genome accession: NZ_CP061778
• Coordinates: 2184696..2185844 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain ZBX-P13
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2179696..2190844

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IFJ79_RS10170 (IFJ79_10170)	-	2180847..2181143 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
IFJ79_RS10175 (IFJ79_10175)	-	2181140..2181733 (-)	594	WP_003084544.1	YggT family protein	-
IFJ79_RS10180 (IFJ79_10180)	proC	2181744..2182565 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
IFJ79_RS10185 (IFJ79_10185)	-	2182577..2183269 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
IFJ79_RS10190 (IFJ79_10190)	pilT	2183484..2184518 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
IFJ79_RS10195 (IFJ79_10195)	pilU	2184696..2185844 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
IFJ79_RS10200 (IFJ79_10200)	czcD	2185852..2186751 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
IFJ79_RS10205 (IFJ79_10205)	-	2186906..2187310 (+)	405	WP_003084560.1	NINE protein	-
IFJ79_RS10210 (IFJ79_10210)	-	2187553..2188926 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
IFJ79_RS10215 (IFJ79_10215)	-	2188923..2190107 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=422689 IFJ79_RS10195 WP_003110016.1 2184696..2185844(+) (pilU) [Pseudomonas aeruginosa strain ZBX-P13]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=422689 IFJ79_RS10195 WP_003110016.1 2184696..2185844(+) (pilU) [Pseudomonas aeruginosa strain ZBX-P13]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATAACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382