Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   G3T13_RS06865 Genome accession   NZ_CP048686
Coordinates   1419716..1420990 (+) Length   424 a.a.
NCBI ID   WP_061773789.1    Uniprot ID   -
Organism   Lactococcus raffinolactis strain KACC 13441     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1414716..1425990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3T13_RS06830 rplA 1415115..1415801 (-) 687 WP_061773794.1 50S ribosomal protein L1 -
  G3T13_RS06835 rplK 1415974..1416399 (-) 426 WP_003139488.1 50S ribosomal protein L11 -
  G3T13_RS06840 - 1416641..1416850 (-) 210 WP_061773793.1 YozE family protein -
  G3T13_RS06845 msrA 1416847..1417368 (-) 522 WP_061773792.1 peptide-methionine (S)-S-oxide reductase MsrA -
  G3T13_RS06850 - 1417365..1418228 (-) 864 WP_096824017.1 S1 RNA-binding domain-containing protein -
  G3T13_RS06860 - 1419049..1419672 (-) 624 WP_061773790.1 YigZ family protein -
  G3T13_RS06865 comFA/cflA 1419716..1420990 (+) 1275 WP_061773789.1 DEAD/DEAH box helicase Machinery gene
  G3T13_RS06870 comFC 1421305..1421634 (+) 330 WP_163604800.1 ComF family protein Machinery gene
  G3T13_RS06875 - 1421637..1422038 (-) 402 WP_061773787.1 diacylglycerol kinase family protein -
  G3T13_RS06880 ybeY 1422022..1422513 (-) 492 WP_061773786.1 rRNA maturation RNase YbeY -
  G3T13_RS06885 - 1422571..1423029 (-) 459 WP_061773785.1 HIT family protein -
  G3T13_RS06890 - 1423022..1424008 (-) 987 WP_061773784.1 PhoH family protein -
  G3T13_RS11475 - 1424005..1424181 (-) 177 WP_003137484.1 hypothetical protein -
  G3T13_RS06895 obgE 1424268..1425587 (-) 1320 WP_082785336.1 GTPase ObgE -

Sequence

Protein


Download         Length: 424 a.a.        Molecular weight: 47655.76 Da        Isoelectric Point: 9.0367

>NTDB_id=422281 G3T13_RS06865 WP_061773789.1 1419716..1420990(+) (comFA/cflA) [Lactococcus raffinolactis strain KACC 13441]
MDNLYCRLLTRQELGGDFENLPKDTETFPGMSITPKSVTCHRCGTTSSLQAVKLEIPAYFCPECLHLGRVRSDELLYHLP
QQDFPPQDSLLWSGQLTPYQAEISKELMAAVDQKTQILVHAVTGAGKTEMIYAAVARVISSGGAVGIATPRTDVARELHA
RLSRDFSIPISLLHAESEPYFPTPLVISTTHQLLRFRHAFDLLIIDEVDAFPYADNDALYFAAEQSRKPTATLIYLTATS
TDKLDKLVKTSQLKQVSLSRRFHGNPLVVPKVIFSGSEKRIYRHIKKQRETGFPLLIFAPVIAFGQSFTERLRRLFPDEK
IGFVASTSEERAEEIDRFRQGQLTILVSTTILERGVTFPKVDVFVVNSHHRLFTKSSLIQIAGRAGRSPDRPTGLVYFFH
TGLTRDMTRAISEIRQMNRLGGFT

Nucleotide


Download         Length: 1275 bp        

>NTDB_id=422281 G3T13_RS06865 WP_061773789.1 1419716..1420990(+) (comFA/cflA) [Lactococcus raffinolactis strain KACC 13441]
ATGGATAACTTATACTGTCGCCTATTAACGCGACAAGAACTGGGGGGTGACTTCGAAAATCTCCCAAAAGACACCGAGAC
CTTCCCTGGGATGTCAATCACACCAAAATCGGTGACTTGCCATCGATGCGGGACAACATCTTCTCTTCAAGCAGTTAAGT
TAGAGATTCCTGCTTACTTTTGTCCAGAGTGCCTTCACCTCGGACGGGTTCGGTCTGATGAATTACTCTACCACTTACCT
CAGCAAGATTTCCCACCTCAAGATTCCCTCCTTTGGAGCGGCCAACTCACGCCCTATCAAGCTGAAATTTCAAAGGAACT
GATGGCTGCAGTTGATCAGAAAACTCAGATTTTAGTCCATGCTGTTACAGGGGCTGGTAAAACTGAGATGATTTATGCTG
CCGTGGCTAGAGTCATCTCAAGTGGTGGGGCCGTTGGGATTGCGACGCCCAGGACCGACGTTGCCCGCGAACTACATGCC
AGATTATCTCGCGACTTTTCTATCCCAATCTCTCTCCTACACGCTGAATCAGAGCCTTACTTTCCGACACCACTCGTCAT
TTCAACCACCCACCAACTACTGCGCTTTCGCCATGCCTTTGATCTATTGATTATCGACGAAGTGGACGCCTTTCCCTACG
CTGATAATGATGCCCTTTACTTTGCGGCTGAGCAGTCCCGAAAACCTACCGCAACGTTGATTTATCTAACCGCGACATCT
ACTGACAAACTCGATAAACTGGTCAAAACTAGCCAGTTAAAACAGGTATCTTTGTCCCGTCGCTTTCATGGCAACCCACT
CGTTGTCCCAAAAGTCATCTTCAGTGGCAGCGAAAAACGCATCTACCGTCACATCAAAAAACAGCGGGAGACAGGTTTTC
CACTCCTCATTTTCGCGCCCGTCATTGCCTTTGGTCAATCGTTCACAGAGCGCTTGCGACGTCTGTTTCCTGACGAAAAA
ATTGGCTTTGTAGCCTCTACTAGTGAGGAGCGTGCTGAGGAGATTGACCGTTTTCGACAAGGGCAACTCACGATTTTAGT
TTCGACGACCATTTTAGAGCGAGGGGTGACTTTTCCTAAGGTTGATGTTTTTGTGGTCAATAGTCACCACCGTCTCTTTA
CCAAATCAAGTTTGATTCAGATTGCGGGAAGGGCTGGCCGAAGCCCTGATCGGCCGACTGGTCTGGTATACTTTTTTCAT
ACTGGCCTAACCAGAGACATGACCCGAGCTATTTCAGAAATTCGCCAGATGAATCGACTGGGAGGTTTTACATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(281-387)

Type III restriction enzyme, res subunit (ResIII)

(95-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis SK321

55.684

100

0.566
  comFA/cflA Streptococcus pneumoniae Rx1

55.738

100

0.561
  comFA/cflA Streptococcus pneumoniae D39

55.738

100

0.561
  comFA/cflA Streptococcus pneumoniae R6

55.738

100

0.561
  comFA/cflA Streptococcus pneumoniae TIGR4

55.504

100

0.559
  comFA/cflA Streptococcus mitis NCTC 12261

54.988

100

0.559
  comFA Lactococcus lactis subsp. cremoris KW2

56.888

92.453

0.526
  comFA Bacillus subtilis subsp. subtilis str. 168

37.019

98.113

0.363

Multiple sequence alignment