Detailed information NTG0422274

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: G3T13_RS04865
• Genome accession: NZ_CP048686
• Coordinates: 1010522..1012768 (-)
• Length: 748 a.a.
• NCBI ID: WP_239519162.1
• Uniprot ID: -
• Organism: Lactococcus raffinolactis strain KACC 13441
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1005522..1017768

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G3T13_RS04835	atpA	1005779..1007284 (-)	1506	WP_061773979.1	F0F1 ATP synthase subunit alpha	-
G3T13_RS04840	-	1007425..1007952 (-)	528	WP_061773978.1	F0F1 ATP synthase subunit delta	-
G3T13_RS04845	atpF	1007955..1008464 (-)	510	WP_061773977.1	F0F1 ATP synthase subunit B	-
G3T13_RS04850	atpB	1008477..1009187 (-)	711	WP_061773976.1	F0F1 ATP synthase subunit A	-
G3T13_RS04855	-	1009293..1009493 (-)	201	WP_003140734.1	F0F1 ATP synthase subunit C	-
G3T13_RS04860	-	1009649..1010443 (-)	795	WP_061773975.1	alpha/beta hydrolase	-
G3T13_RS04865	comEC	1010522..1012768 (-)	2247	WP_239519162.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
G3T13_RS04870	-	1012734..1013387 (-)	654	WP_061773974.1	helix-hairpin-helix domain-containing protein	-
G3T13_RS04875	-	1013445..1014665 (-)	1221	WP_061773973.1	ABC transporter permease	-
G3T13_RS04880	-	1014658..1015551 (-)	894	WP_061773972.1	ABC transporter ATP-binding protein	-
G3T13_RS04885	-	1015726..1016160 (-)	435	WP_061773971.1	hypothetical protein	-
G3T13_RS04890	-	1016216..1016410 (-)	195	WP_061773970.1	helix-turn-helix transcriptional regulator	-

Sequence

Protein

• Length: 748 a.a.
• Molecular weight: 85379.44 Da
• Isoelectric Point: 10.1525

>NTDB_id=422274 G3T13_RS04865 WP_239519162.1 1010522..1012768(-) (comEC) [Lactococcus raffinolactis strain KACC 13441]
MKNLKKALRWINLPSIFIAYFCVLIYYTVFCFSYLLLGYLIISLLILIWQNGIRTCLKLSMILIGFATICLALQDREAKD
FAREMTVTRISPILDTIQVDGDQVSFRGKSGKQTYQVYYRVTTEQEQRYFKQLAQPVVLSISGQLEKASQQRNFNGFDYQ
HHLKTQNIYRVLTIEQVTGVTRKQTLTLALLRRKAILFSEQHFPRPMSAYMTGLLFGHLGKDFEDMSSIYTSLGIMHLFA
LSGMQVSFFIDFLRKGMLRLGFRRDVVDWFQIPFSVFYAGLTGFSISVNRSLVQKILANFGIKALDNFSLTLLLLFITAP
KFLLTTGGTLSLLFAFVISIFGDRFENLPKYRKLLAESLTLSLCVLPLLILYFHTFQPVSIILTFVFSFLFDILFLPGLS
VIFLLAMVTGFTLTQVNLIFQWLEDLIKLTDSWWHYPLVLGKPTALVFLVTLVVTVIDHWRNRKVSARLAIILMGLFFIT
KNPPTPSITMVDVGQGDSIFLQDKFNRRNILIDTGGRIELGKKKKWQERKIAAPAEKTLIPYLKSRGVGQIDTLVLTHTH
EDHMGDLLAVIDQIKVKNIIVSQGSLNNAQFVKLLKQTRAKVQVAKVGQRLKIFDSYLEVLYPLTPGDGKNNDSIVLYGK
LYGTKFLLTGDLEETGERELLTRYPNLSVDVLKVGHHGSKTSSSESFIEAIRPKVGLISCGLDNRYGHPNMETLTTFQKY
EVQTFRTDYHGAIKFKKNSKFWHISTVK

Nucleotide

• Length: 2247 bp

>NTDB_id=422274 G3T13_RS04865 WP_239519162.1 1010522..1012768(-) (comEC) [Lactococcus raffinolactis strain KACC 13441]
TTGAAAAACTTAAAGAAAGCATTACGGTGGATTAATCTACCATCGATTTTCATCGCCTATTTTTGTGTGCTGATCTACTA
TACCGTCTTTTGTTTCTCCTATTTACTTTTAGGTTATCTCATCATTTCTTTGCTAATCTTGATCTGGCAAAATGGCATCA
GAACTTGTTTAAAGCTCAGCATGATCTTAATTGGGTTTGCAACCATTTGTCTAGCGCTACAAGATCGTGAAGCTAAAGAT
TTTGCGCGTGAGATGACAGTGACACGGATCAGCCCCATACTGGATACGATTCAGGTGGATGGTGACCAGGTCTCTTTTCG
TGGGAAATCGGGCAAACAAACCTATCAAGTCTATTATAGGGTCACGACGGAACAAGAGCAGCGCTACTTTAAGCAATTAG
CACAACCAGTCGTCCTATCCATTTCAGGTCAACTAGAAAAAGCGAGTCAACAAAGAAATTTCAATGGCTTTGATTATCAA
CACCATCTCAAGACACAAAATATCTATCGAGTTTTGACGATTGAACAGGTGACAGGGGTGACAAGAAAACAAACCTTAAC
CTTAGCGCTCTTACGACGAAAAGCTATTTTGTTTAGTGAGCAACACTTTCCGCGACCTATGTCAGCCTACATGACCGGTT
TACTTTTTGGGCATCTCGGTAAAGATTTTGAAGATATGAGCAGTATCTATACCAGCTTAGGCATCATGCACCTCTTTGCG
CTATCAGGCATGCAAGTGTCATTTTTTATCGACTTTTTAAGAAAGGGTATGCTCAGACTAGGGTTCAGGAGAGATGTTGT
CGATTGGTTTCAAATCCCTTTTTCTGTTTTTTATGCTGGTCTTACAGGATTTTCCATATCTGTCAACAGATCCTTGGTCC
AGAAAATCCTAGCTAATTTTGGAATTAAGGCGCTTGACAATTTCAGTCTCACGTTGTTGCTTCTATTTATCACGGCGCCA
AAATTCCTTTTAACCACAGGCGGCACCTTGTCTTTACTTTTTGCCTTCGTCATTTCAATCTTTGGTGATCGCTTTGAGAA
CCTCCCAAAATATCGTAAACTACTAGCAGAAAGTTTAACCCTTTCTCTATGCGTTTTACCGCTCCTCATCCTTTATTTTC
ACACCTTCCAACCAGTGTCGATCATCTTGACCTTTGTCTTCAGCTTTCTTTTTGATATTCTCTTCCTACCAGGTCTATCC
GTCATTTTCTTGCTAGCAATGGTAACAGGCTTCACGCTAACACAAGTCAATCTCATTTTTCAGTGGCTTGAAGACCTTAT
CAAATTGACAGATAGCTGGTGGCATTATCCGTTAGTACTTGGTAAACCAACAGCCTTGGTCTTTCTGGTGACGCTAGTTG
TGACAGTAATTGACCACTGGCGAAATCGAAAAGTTAGTGCTAGGCTGGCAATCATCTTGATGGGCTTATTCTTCATAACT
AAAAATCCACCGACTCCTTCGATAACCATGGTGGATGTTGGCCAGGGGGATAGTATTTTCTTACAAGATAAGTTTAATCG
ACGCAATATTTTAATCGATACAGGTGGCAGGATTGAACTAGGTAAGAAGAAAAAGTGGCAGGAGCGTAAAATTGCAGCAC
CAGCTGAAAAGACTTTAATTCCTTATTTGAAAAGTCGGGGTGTGGGTCAGATTGATACTTTGGTTCTAACCCATACTCAC
GAGGACCATATGGGTGACCTACTCGCTGTCATCGACCAAATTAAGGTCAAAAATATCATCGTGAGTCAAGGCAGTCTCAA
TAATGCCCAATTTGTCAAACTCTTAAAGCAAACCCGAGCTAAGGTTCAAGTGGCTAAGGTCGGTCAACGACTAAAGATTT
TTGACAGCTATTTGGAAGTTCTGTATCCATTGACCCCAGGAGATGGTAAAAATAATGACTCGATCGTCTTGTATGGTAAG
CTCTATGGGACAAAATTTTTGTTAACAGGTGATTTGGAGGAGACAGGCGAGCGAGAGTTGTTGACACGTTATCCCAATCT
CTCAGTTGATGTTTTAAAAGTAGGGCATCATGGCTCAAAAACGTCATCTTCTGAGTCTTTTATTGAAGCGATTCGACCAA
AAGTCGGATTGATTTCTTGTGGTTTGGATAATCGGTACGGTCATCCAAATATGGAAACTCTCACAACCTTCCAGAAATAT
GAGGTTCAAACTTTTCGAACAGATTATCATGGTGCTATAAAATTTAAGAAAAACAGTAAATTTTGGCACATCTCGACAGT
AAAGTGA

Domains

Predicted by InterproScan.

• Competence protein (Competence) (214-456)

• Domain of unknown function (DUF4131) (DUF4131) (34-178)

• Metallo-beta-lactamase superfamily (Lactamase_B) (493-701)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Lactococcus lactis subsp. cremoris KW2	51.957	99.064	0.515
comEC/celB	Streptococcus mitis NCTC 12261	47.215	100	0.476
comEC/celB	Streptococcus mitis SK321	46.631	100	0.472
comEC/celB	Streptococcus pneumoniae TIGR4	45.046	100	0.456
comEC/celB	Streptococcus pneumoniae Rx1	44.254	100	0.448
comEC/celB	Streptococcus pneumoniae D39	44.254	100	0.448
comEC/celB	Streptococcus pneumoniae R6	44.254	100	0.448