Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BURJV3_RS04760 Genome accession   NC_015947
Coordinates   1050220..1051383 (+) Length   387 a.a.
NCBI ID   WP_042358565.1    Uniprot ID   A0A6N7NYV7
Organism   Stenotrophomonas maltophilia JV3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1045220..1056383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BURJV3_RS04730 (BurJV3_0921) soxR 1045359..1045793 (+) 435 WP_014036217.1 redox-sensitive transcriptional activator SoxR -
  BURJV3_RS04735 (BurJV3_0922) - 1045860..1046462 (-) 603 WP_014036218.1 short chain dehydrogenase -
  BURJV3_RS04740 (BurJV3_0923) - 1046563..1047438 (+) 876 WP_014036219.1 LysR family transcriptional regulator -
  BURJV3_RS04745 (BurJV3_0924) proC 1047499..1048320 (-) 822 WP_014036220.1 pyrroline-5-carboxylate reductase -
  BURJV3_RS04750 (BurJV3_0925) - 1048339..1049016 (-) 678 WP_014036221.1 YggS family pyridoxal phosphate-dependent enzyme -
  BURJV3_RS04755 (BurJV3_0926) pilT 1049104..1050141 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  BURJV3_RS04760 (BurJV3_0927) pilU 1050220..1051383 (+) 1164 WP_042358565.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BURJV3_RS04765 (BurJV3_0928) - 1051470..1052147 (+) 678 WP_014036223.1 hypothetical protein -
  BURJV3_RS04770 (BurJV3_0929) - 1052258..1053241 (+) 984 WP_014036224.1 aldo/keto reductase -
  BURJV3_RS04775 (BurJV3_0930) - 1053326..1054222 (-) 897 WP_014036225.1 LysR family transcriptional regulator -
  BURJV3_RS04780 (BurJV3_0931) - 1054317..1054526 (+) 210 WP_014036226.1 DUF4287 domain-containing protein -
  BURJV3_RS04785 (BurJV3_0932) - 1054477..1054926 (+) 450 WP_232289682.1 GNAT family N-acetyltransferase -
  BURJV3_RS04790 (BurJV3_0933) - 1055020..1055727 (+) 708 WP_014036228.1 YitT family protein -
  BURJV3_RS04795 (BurJV3_0934) - 1055801..1056343 (-) 543 WP_014036229.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43108.51 Da        Isoelectric Point: 6.5899

>NTDB_id=42153 BURJV3_RS04760 WP_042358565.1 1050220..1051383(+) (pilU) [Stenotrophomonas maltophilia JV3]
MPSWEPAVNSTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKT
HECNFAIGLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGY
RNQNSTGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLC
TLHANNANQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVM
KDSVQLGMKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=42153 BURJV3_RS04760 WP_042358565.1 1050220..1051383(+) (pilU) [Stenotrophomonas maltophilia JV3]
ATGCCCTCTTGGGAGCCCGCCGTGAACAGCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCA
GCGCGCCTCGGACCTGTTCATCACGGCCGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACGCAGA
CCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACC
CACGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGTGTCAGCTGCTTCTACCAGCGCAACCAGGTCGG
CATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGA
TGACCAAGCGCGGCATCATCCTGTTCGTCGGCGCCACCGGTACCGGTAAATCGACCTCGCTGGCAGCGATGATCGGTTAC
CGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCAT
CACCCAGCGCGAAGTCGGCATCGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAAGCGCCGGACGTGA
TCATGATCGGCGAGGTGCGTACCCGCGAAGGCATGGATCACGCCATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGTGC
ACCCTGCACGCGAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGCAACCAGTTGCT
GATGGACCTGTCGCTGAACCTCAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCGGATGGCCGCTCGCGCAAGGTGG
CGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATG
AAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGA
GGACGCGCTGCGCTACGCCGATTCGCAGAACGAAGTACGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCGCGCACCC
TGTCGCAGGGGCTGGATGGCGTGGAGATTTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N7NYV7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

94.832

0.643
  pilU Acinetobacter baylyi ADP1

63.585

92.248

0.587
  pilU Vibrio cholerae strain A1552

53.371

91.99

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

91.731

0.395

Multiple sequence alignment