Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   ECO55CA74_RS11600 Genome accession   NC_017656
Coordinates   2296120..2297130 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli O55:H7 str. RM12579     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2291120..2302130
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECO55CA74_RS11580 (ECO55CA74_11110) mepM 2291988..2293310 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  ECO55CA74_RS11585 (ECO55CA74_11115) znuA 2293326..2294258 (-) 933 WP_001417981.1 zinc ABC transporter substrate-binding protein ZnuA -
  ECO55CA74_RS11590 (ECO55CA74_11120) znuC 2294337..2295092 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  ECO55CA74_RS11595 (ECO55CA74_11125) znuB 2295089..2295874 (+) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  ECO55CA74_RS11600 (ECO55CA74_11130) ruvB 2296120..2297130 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  ECO55CA74_RS11605 (ECO55CA74_11135) ruvA 2297139..2297750 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  ECO55CA74_RS30280 yobI 2297889..2297954 (-) 66 WP_010723105.1 stress response small protein YobI -
  ECO55CA74_RS11610 (ECO55CA74_11140) yebB 2298026..2298628 (+) 603 WP_001024953.1 YebB family permuted papain-like enzyme -
  ECO55CA74_RS11615 (ECO55CA74_11145) ruvC 2298630..2299151 (-) 522 WP_001419096.1 crossover junction endodeoxyribonuclease RuvC -
  ECO55CA74_RS11620 (ECO55CA74_11150) yebC 2299186..2299926 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  ECO55CA74_RS11625 (ECO55CA74_11155) nudB 2299955..2300407 (-) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=42124 ECO55CA74_RS11600 WP_000568519.1 2296120..2297130(-) (ruvB) [Escherichia coli O55:H7 str. RM12579]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=42124 ECO55CA74_RS11600 WP_000568519.1 2296120..2297130(-) (ruvB) [Escherichia coli O55:H7 str. RM12579]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTTTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment