Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   D8K17_RS12150 Genome accession   NZ_CP061322
Coordinates   2384431..2385432 (-) Length   333 a.a.
NCBI ID   WP_003131994.1    Uniprot ID   Q9CDL3
Organism   Lactococcus lactis subsp. lactis bv. diacetylactis strain S50     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2379431..2390432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8K17_RS12115 (D8K17_012140) - 2379720..2380085 (-) 366 WP_003131985.1 MerR family transcriptional regulator -
  D8K17_RS12120 (D8K17_012145) - 2380186..2380623 (-) 438 WP_010906378.1 low molecular weight protein-tyrosine-phosphatase -
  D8K17_RS12125 (D8K17_012150) - 2380767..2380931 (-) 165 WP_003131988.1 hypothetical protein -
  D8K17_RS12130 (D8K17_012155) - 2381036..2381653 (-) 618 WP_010906379.1 AAA family ATPase -
  D8K17_RS12135 (D8K17_012160) - 2381650..2382834 (-) 1185 WP_010906380.1 ABC transporter permease -
  D8K17_RS12145 (D8K17_012170) - 2383410..2384294 (-) 885 WP_010906381.1 XRE/MutR family transcriptional regulator -
  D8K17_RS12150 (D8K17_012175) ruvB 2384431..2385432 (-) 1002 WP_003131994.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  D8K17_RS12155 (D8K17_012180) ruvA 2385558..2386151 (-) 594 WP_003131995.1 Holliday junction branch migration protein RuvA Machinery gene
  D8K17_RS12160 (D8K17_012185) hexB 2386264..2388234 (-) 1971 WP_031297070.1 DNA mismatch repair endonuclease MutL Machinery gene
  D8K17_RS12165 (D8K17_012190) - 2388363..2389058 (-) 696 WP_010906384.1 hypothetical protein -

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 37633.17 Da        Isoelectric Point: 4.5825

>NTDB_id=421102 D8K17_RS12150 WP_003131994.1 2384431..2385432(-) (ruvB) [Lactococcus lactis subsp. lactis bv. diacetylactis strain S50]
MNDILNKEPMEEDYGIEKSLRPQFFNQYIGQDKVKEQLEIFIKAAKMREEVLDHVLLFGPPGLGKTTMAFVIANELGVNI
KQTAGPAIEKPGDLVAILNELEPGDVLFIDEIHRMPMQVEEVLYSAMEDFYIDIMLGSGDGSRSVHLDLPPFTLVGATTR
AGMLSNPLRARFGISSHMEYYQERDLEEIVKRTADIFEVEVIDNAALEIALRSRGTPRIANRLLKRVRDFAQIMGDGRVD
KAITDKALSILDVDAAGLDYIDQKILRTMIEMYHGGPVGIGTLAVNIAEDRETVEDMYEPYLIQKGFLMRTKQGRKVTQR
AYEHLGYVYNEED

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=421102 D8K17_RS12150 WP_003131994.1 2384431..2385432(-) (ruvB) [Lactococcus lactis subsp. lactis bv. diacetylactis strain S50]
ATGAACGATATATTAAACAAAGAGCCAATGGAAGAAGATTACGGTATTGAAAAATCTCTACGTCCGCAATTTTTTAATCA
ATATATTGGTCAAGATAAAGTCAAAGAACAATTAGAAATCTTTATTAAAGCAGCAAAAATGCGTGAAGAAGTTTTGGACC
ACGTGTTACTTTTTGGCCCTCCGGGACTTGGTAAGACAACAATGGCATTTGTCATTGCCAATGAGTTGGGAGTTAATATT
AAACAAACAGCGGGTCCTGCCATTGAAAAGCCAGGAGATTTAGTTGCAATTTTGAATGAATTGGAACCTGGCGATGTACT
ATTTATTGATGAAATTCATCGGATGCCAATGCAAGTTGAAGAAGTTCTTTATAGCGCTATGGAAGATTTCTACATTGATA
TTATGCTTGGTTCTGGTGATGGTTCACGCTCGGTTCATTTAGATTTGCCGCCATTTACTCTAGTTGGGGCAACAACAAGA
GCTGGAATGCTTTCGAATCCATTGCGTGCCCGTTTTGGAATTTCAAGTCATATGGAGTATTATCAAGAGCGTGATTTAGA
AGAAATTGTTAAAAGAACAGCTGATATTTTTGAAGTCGAAGTTATTGATAATGCCGCTTTAGAGATTGCTTTACGTAGTC
GAGGGACTCCACGGATTGCCAATCGTTTACTTAAACGAGTGCGTGATTTTGCTCAAATAATGGGGGATGGCCGAGTGGAT
AAAGCAATTACCGATAAGGCCTTGTCTATTCTTGATGTTGATGCCGCTGGTTTGGACTATATTGACCAAAAAATTCTCCG
CACAATGATTGAAATGTACCACGGTGGTCCTGTTGGGATTGGAACTTTGGCGGTAAATATTGCCGAAGACCGTGAAACGG
TCGAGGATATGTATGAACCTTATTTGATTCAAAAAGGATTTTTGATGAGAACTAAACAGGGACGTAAGGTGACCCAAAGA
GCCTATGAACACCTAGGGTATGTTTACAATGAAGAAGATTGA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(256-325)

RuvB AAA lid domain (AAA_lid_4)

(181-254)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(20-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CDL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Streptococcus pneumoniae R6

78.614

99.7

0.784
  ruvB Streptococcus pneumoniae D39

78.614

99.7

0.784
  ruvB Streptococcus pneumoniae TIGR4

78.313

99.7

0.781
  ruvB Bacillus subtilis subsp. subtilis str. 168

61.491

96.697

0.595
  ruvB Helicobacter pylori 26695

51.242

96.697

0.495
  ruvB Synechocystis sp. PCC 6803

51.613

93.093

0.48

Multiple sequence alignment