Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   GV127_RS10260 Genome accession   NZ_CP048402
Coordinates   2205038..2206372 (+) Length   444 a.a.
NCBI ID   WP_061406404.1    Uniprot ID   -
Organism   Pasteurella multocida strain HS_Canada1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2200038..2211372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GV127_RS10235 (GV127_10235) - 2200114..2202675 (-) 2562 WP_163884264.1 penicillin-binding protein 1A -
  GV127_RS10240 (GV127_10240) - 2202811..2203584 (+) 774 WP_046333757.1 pilus assembly protein PilM -
  GV127_RS10245 (GV127_10245) - 2203624..2204139 (+) 516 WP_005751904.1 hypothetical protein -
  GV127_RS10250 (GV127_10250) - 2204139..2204663 (+) 525 WP_005751903.1 hypothetical protein -
  GV127_RS10255 (GV127_10255) - 2204666..2205028 (+) 363 WP_005723703.1 pilus assembly protein PilP -
  GV127_RS10260 (GV127_10260) comE 2205038..2206372 (+) 1335 WP_061406404.1 type IV pilus secretin PilQ Machinery gene
  GV127_RS10265 (GV127_10265) aroK 2206555..2207082 (+) 528 WP_005717623.1 shikimate kinase AroK -
  GV127_RS10270 (GV127_10270) aroB 2207099..2208187 (+) 1089 WP_163884265.1 3-dehydroquinate synthase -
  GV127_RS10275 (GV127_10275) - 2208191..2209096 (+) 906 WP_005757382.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  GV127_RS10280 (GV127_10280) - 2209143..2209448 (-) 306 WP_005723691.1 DUF721 domain-containing protein -
  GV127_RS10285 (GV127_10285) secM 2209541..2209852 (+) 312 WP_005723689.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49240.64 Da        Isoelectric Point: 8.0315

>NTDB_id=421056 GV127_RS10260 WP_061406404.1 2205038..2206372(+) (comE) [Pasteurella multocida strain HS_Canada1]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IARMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=421056 GV127_RS10260 WP_061406404.1 2205038..2206372(+) (comE) [Pasteurella multocida strain HS_Canada1]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAGAATGAAACAACTTGAGTTACATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGAGGTGGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
TAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCAGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACTCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.037

97.297

0.604
  comE Haemophilus influenzae 86-028NP

61.343

97.297

0.597
  comE Glaesserella parasuis strain SC1401

48.961

97.523

0.477
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.806

92.793

0.369
  pilQ Vibrio cholerae strain A1552

39.806

92.793

0.369

Multiple sequence alignment