Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   M654_RS16300 Genome accession   NZ_CP048273
Coordinates   3121500..3122129 (-) Length   209 a.a.
NCBI ID   WP_026587937.1    Uniprot ID   -
Organism   Bacillus sp. NSP9.1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3116500..3127129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M654_RS16280 (M654_016230) holA 3117025..3118047 (-) 1023 WP_026587933.1 DNA polymerase III subunit delta -
  M654_RS16285 (M654_016235) - 3118282..3118413 (+) 132 WP_026587934.1 YqzM family protein -
  M654_RS16290 (M654_016240) comEC 3118525..3120855 (-) 2331 WP_026587935.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  M654_RS16295 (M654_016245) - 3120862..3121431 (-) 570 WP_026587936.1 ComE operon protein 2 -
  M654_RS16300 (M654_016250) comEA 3121500..3122129 (-) 630 WP_026587937.1 helix-hairpin-helix domain-containing protein Machinery gene
  M654_RS16305 (M654_016255) comER 3122192..3123034 (+) 843 WP_256860380.1 late competence protein ComER -
  M654_RS16310 (M654_016260) - 3123102..3123857 (-) 756 WP_026587939.1 class I SAM-dependent methyltransferase -
  M654_RS16315 (M654_016265) rsfS 3123857..3124210 (-) 354 WP_026587940.1 ribosome silencing factor -
  M654_RS16320 (M654_016270) yqeK 3124224..3124787 (-) 564 WP_026587941.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  M654_RS16325 (M654_016275) - 3124777..3125346 (-) 570 WP_026587942.1 nicotinate-nucleotide adenylyltransferase -
  M654_RS16330 (M654_016280) yhbY 3125367..3125657 (-) 291 WP_026587943.1 ribosome assembly RNA-binding protein YhbY -
  M654_RS16335 (M654_016285) aroE 3125651..3126487 (-) 837 WP_026587944.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22349.34 Da        Isoelectric Point: 5.0051

>NTDB_id=420467 M654_RS16300 WP_026587937.1 3121500..3122129(-) (comEA) [Bacillus sp. NSP9.1]
MLNWLKQYKWFAAGGLALVVIIAAAVVTKGGDDLSSAHSSVPDEALQSPEKTASLPEDKKNEEKEEIVIDLKGAVKQPGV
YEMKAGDRVHHLLKKAGGTVKKADQKQVNLAAILRDGMVVYIPFQGEEASAHSESGAYLAEGEAENMININTASAKELEG
IPGVGPSKAEAIVTYREENGPFQTIEDITNVSGIGEKSFEKIKSSISVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=420467 M654_RS16300 WP_026587937.1 3121500..3122129(-) (comEA) [Bacillus sp. NSP9.1]
ATGCTAAATTGGCTCAAACAATACAAATGGTTTGCGGCAGGGGGATTGGCGCTAGTGGTCATCATTGCCGCCGCAGTCGT
CACAAAGGGCGGTGACGATCTATCCTCAGCTCATTCGAGTGTGCCGGATGAAGCTTTGCAATCACCTGAGAAGACGGCAA
GTTTGCCAGAGGATAAGAAAAACGAAGAAAAAGAAGAAATCGTCATCGATCTGAAGGGGGCGGTCAAGCAGCCCGGTGTT
TACGAAATGAAGGCAGGTGACAGAGTTCATCATTTACTAAAAAAAGCGGGCGGCACCGTGAAAAAAGCCGATCAAAAACA
AGTCAACCTAGCCGCCATTTTACGGGACGGCATGGTCGTTTATATTCCGTTTCAAGGAGAAGAAGCCTCCGCTCATTCTG
AATCAGGGGCGTATTTGGCAGAAGGAGAAGCGGAAAACATGATCAATATCAACACAGCTTCTGCAAAAGAGCTTGAGGGC
ATTCCCGGTGTTGGCCCTTCAAAAGCGGAAGCGATCGTCACATATCGGGAGGAAAACGGCCCATTTCAGACGATAGAAGA
CATCACCAATGTGTCAGGTATTGGAGAAAAATCATTTGAAAAAATAAAATCGTCAATATCGGTAAAGTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(69-123)

Helix-hairpin-helix motif (HHH_3)

(145-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

53.11

100

0.531
  comEA Staphylococcus aureus MW2

35.616

100

0.373
  comEA Staphylococcus aureus N315

35.321

100

0.368

Multiple sequence alignment