Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   M654_RS03425 Genome accession   NZ_CP048273
Coordinates   706595..707983 (+) Length   462 a.a.
NCBI ID   WP_026588944.1    Uniprot ID   A0A6I7FGU1
Organism   Bacillus sp. NSP9.1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 701595..712983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M654_RS03400 (M654_003395) - 701620..702696 (-) 1077 WP_026588950.1 LCP family protein -
  M654_RS03405 (M654_003400) - 702710..703348 (-) 639 WP_026588949.1 YigZ family protein -
  M654_RS03410 (M654_003405) degS 703570..704727 (+) 1158 WP_026588948.1 histidine kinase Regulator
  M654_RS03415 (M654_003410) degU 704809..705498 (+) 690 WP_026588947.1 two-component system response regulator DegU Regulator
  M654_RS03420 (M654_003415) - 705618..706460 (+) 843 WP_026588946.1 DegV family protein -
  M654_RS03425 (M654_003420) comFA 706595..707983 (+) 1389 WP_026588944.1 DEAD/DEAH box helicase Machinery gene
  M654_RS03430 (M654_003425) - 708040..708324 (+) 285 WP_026588943.1 competence protein ComFB -
  M654_RS03435 (M654_003430) comFC 708281..709015 (+) 735 WP_035428518.1 ComF family protein Machinery gene
  M654_RS03440 (M654_003435) - 709074..709493 (+) 420 WP_026588941.1 TIGR03826 family flagellar region protein -
  M654_RS03445 (M654_003440) flgM 709574..709840 (+) 267 WP_026588940.1 flagellar biosynthesis anti-sigma factor FlgM -
  M654_RS03450 (M654_003445) - 709860..710339 (+) 480 WP_026588939.1 flagellar protein FlgN -
  M654_RS03455 (M654_003450) flgK 710355..711878 (+) 1524 WP_026588938.1 flagellar hook-associated protein FlgK -
  M654_RS03460 (M654_003455) flgL 711889..712800 (+) 912 WP_026588937.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 51847.71 Da        Isoelectric Point: 9.8759

>NTDB_id=420424 M654_RS03425 WP_026588944.1 706595..707983(+) (comFA) [Bacillus sp. NSP9.1]
MEHEASAYTGNLQALLEARQLLKSELPFPDSAVEWHIQNGLIKAETPIQKTAKGLICMRCGQRDPSRFAQYPCLICQKDC
VYCRFCVMMGRVSECTPLLTWTGGGSSAWPPVSMTWKGVLSAGQEKAARMIVDAIRQKEQLLVWAVCGSGKTEILFQGIE
FALNKGLRVCIATPRTDVVLELAPRLQTAFKGLDIAVLYGGSPDRGKLAPLVISTTHQLLRYKKAFDVIIIDEVDAFPFS
LDQTLQYAVQKARKPQSARIYLTATPSEEMKRSAENGKLRTVQIPSRYHRKPLPEPVMRWCGNWKRGLDKGKLPPVIMQW
LFKHKELQQPVFLFVPSIPILKSVSFLLKKAQFQVESVYADDPGRNEKVGLFREEKLDVLVTTTILERGVTVRKVQVGVL
GAESAVFSESALVQIAGRAGRHPQFPWGYVCFFHFGKTKSMNAACRHIRDMNKLAKKEILVD

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=420424 M654_RS03425 WP_026588944.1 706595..707983(+) (comFA) [Bacillus sp. NSP9.1]
ATCGAACATGAGGCATCCGCATATACCGGCAACCTGCAGGCTCTTCTGGAGGCGCGCCAGCTTCTGAAAAGCGAGCTTCC
CTTTCCGGACAGCGCCGTAGAATGGCATATACAAAACGGACTCATCAAGGCTGAAACACCGATTCAAAAAACGGCAAAGG
GCTTGATCTGCATGAGGTGCGGCCAGCGCGATCCGTCACGTTTTGCGCAATATCCTTGTTTAATATGCCAAAAAGACTGT
GTATACTGCCGTTTCTGCGTAATGATGGGAAGGGTGAGCGAATGCACGCCGCTTTTGACATGGACAGGCGGCGGTTCTTC
GGCATGGCCGCCGGTCAGCATGACCTGGAAAGGTGTTCTTTCGGCCGGACAGGAAAAAGCGGCGCGAATGATTGTCGATG
CCATCCGCCAAAAAGAGCAGCTTTTAGTCTGGGCGGTTTGCGGTTCAGGCAAAACAGAAATACTTTTTCAAGGAATTGAG
TTTGCGCTAAACAAAGGTTTAAGGGTATGCATCGCCACGCCGAGAACGGATGTAGTGCTGGAGCTCGCCCCGCGGCTGCA
GACCGCATTTAAAGGATTGGACATCGCCGTATTATATGGCGGAAGTCCGGACAGAGGGAAGCTTGCACCGCTTGTCATCT
CAACAACCCATCAGCTTCTCCGCTATAAAAAAGCATTTGATGTGATCATCATCGATGAAGTCGACGCTTTTCCATTTTCT
CTTGACCAAACACTGCAATATGCTGTTCAAAAAGCGCGAAAACCGCAAAGCGCCCGCATCTATTTAACCGCCACCCCATC
GGAAGAAATGAAACGAAGCGCTGAAAATGGCAAGCTCCGCACTGTGCAGATACCCTCCAGATATCACCGCAAGCCATTGC
CTGAGCCTGTTATGAGGTGGTGCGGGAACTGGAAGAGAGGACTCGACAAGGGAAAACTGCCGCCCGTAATAATGCAATGG
CTTTTCAAGCATAAAGAGCTGCAGCAGCCGGTTTTTTTATTTGTTCCCTCGATTCCAATTCTCAAATCAGTGTCATTCCT
ATTAAAAAAAGCGCAATTCCAAGTCGAGAGCGTTTATGCAGACGACCCCGGCAGAAATGAGAAAGTCGGGCTGTTCAGAG
AAGAAAAGCTTGATGTACTGGTGACGACAACCATTTTGGAAAGGGGAGTGACGGTCAGAAAAGTCCAGGTCGGGGTGCTC
GGTGCGGAGTCGGCCGTCTTTAGCGAAAGCGCGCTTGTCCAAATCGCCGGCAGAGCCGGCAGACACCCGCAGTTTCCTTG
GGGATACGTCTGTTTTTTTCACTTTGGCAAAACAAAGAGCATGAATGCGGCCTGCCGCCATATTCGCGATATGAATAAAT
TGGCTAAAAAGGAAATATTGGTTGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(319-422)

Type III restriction enzyme, res subunit (ResIII)

(120-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I7FGU1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

59.071

97.835

0.578
  comFA Latilactobacillus sakei subsp. sakei 23K

41.235

87.662

0.361

Multiple sequence alignment