Detailed information NTG0420201

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GYN05_RS08510
• Genome accession: NZ_CP048250
• Coordinates: 1635360..1636220 (+)
• Length: 286 a.a.
• NCBI ID: WP_003689814.1
• Uniprot ID: A0AA44U9R8
• Organism: Neisseria gonorrhoeae strain CT532
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1630360..1641220

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GYN05_RS08495 (GYN05_08265)	dapD	1630870..1631691 (-)	822	WP_003689804.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
GYN05_RS08500 (GYN05_08270)	pgi	1631901..1633544 (-)	1644	WP_003689806.1	glucose-6-phosphate isomerase	-
GYN05_RS08505 (GYN05_08275)	pilG	1634054..1635286 (+)	1233	WP_003689811.1	type 4 pilus assembly protein PilG	Machinery gene
GYN05_RS08510 (GYN05_08280)	pilD	1635360..1636220 (+)	861	WP_003689814.1	prepilin peptidase	Machinery gene
GYN05_RS08515 (GYN05_08285)	coaE	1636217..1636849 (+)	633	WP_003689816.1	dephospho-CoA kinase	-
GYN05_RS08520 (GYN05_08290)	-	1636842..1637051 (+)	210	WP_003696096.1	DNA gyrase inhibitor YacG	-
GYN05_RS08525 (GYN05_08295)	pilF	1637137..1638813 (-)	1677	WP_003689817.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GYN05_RS08535 (GYN05_08305)	-	1639151..1639597 (-)	447	WP_003689818.1	DUF441 domain-containing protein	-
GYN05_RS08540 (GYN05_08310)	-	1639601..1640575 (-)	975	WP_003689819.1	polyprenyl synthetase family protein	-
GYN05_RS08545 (GYN05_08320)	rplU	1640799..1641107 (+)	309	WP_003691838.1	50S ribosomal protein L21	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31332.34 Da
• Isoelectric Point: 8.9561

>NTDB_id=420201 GYN05_RS08510 WP_003689814.1 1635360..1636220(+) (pilD) [Neisseria gonorrhoeae strain CT532]
MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVFAKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVR

Nucleotide

• Length: 861 bp

>NTDB_id=420201 GYN05_RS08510 WP_003689814.1 1635360..1636220(+) (pilD) [Neisseria gonorrhoeae strain CT532]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCAGTTTTGGGGCTGCTGGTCGGCAGCTTCCTGAA
TGTCGTCATTTACCGCGTACCCGTTATGATGGAACGCGGCTGGACGGTATTTGCCAAAGAACATTTAAACCTGCCGCTGA
CCGACGATGAAAGCCGTACCTTCAACCTGATGAAGCCGGATTCCTGCTGTCCCAAATGCCGTGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGTTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTGTTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACGCTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACATTACCC
TTGATCTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATAGTTCATTATGGCTCTTATGTGCAGTGTATAAACTGCTCACAGGAAAAACCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCTCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCGGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (134-242)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	100	100	1
pilD	Vibrio cholerae strain A1552	44.792	100	0.451
pilD	Vibrio campbellii strain DS40M4	45.652	96.503	0.441
pilD	Acinetobacter baumannii D1279779	47.727	92.308	0.441
pilD	Acinetobacter nosocomialis M2	47.727	92.308	0.441