Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GW535_RS19030 Genome accession   NZ_CP048066
Coordinates   298366..299319 (-) Length   317 a.a.
NCBI ID   WP_210428201.1    Uniprot ID   -
Organism   Piscirickettsia salmonis strain Ps-8079A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 291456..297632 298366..299319 flank 734


Gene organization within MGE regions


Location: 291456..299319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GW535_RS01560 (GW535_01550) - 291456..292430 (-) 975 WP_155047246.1 IS30 family transposase -
  GW535_RS01565 (GW535_01555) - 292523..292951 (+) 429 Protein_314 IS701 family transposase -
  GW535_RS19445 - 293350..293520 (+) 171 WP_155046934.1 hypothetical protein -
  GW535_RS01570 (GW535_01560) - 293669..293806 (-) 138 WP_162034402.1 hypothetical protein -
  GW535_RS01575 (GW535_01565) - 293894..294976 (+) 1083 WP_054300162.1 IS481 family transposase -
  GW535_RS01585 (GW535_01575) - 296479..297632 (+) 1154 WP_129556499.1 IS3 family transposase -
  GW535_RS01590 (GW535_01580) - 297674..298096 (+) 423 WP_155046935.1 hypothetical protein -
  GW535_RS19030 pilA 298366..299319 (-) 954 WP_210428201.1 signal recognition particle-docking protein FtsY Machinery gene

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 34389.34 Da        Isoelectric Point: 5.9299

>NTDB_id=419756 GW535_RS19030 WP_210428201.1 298366..299319(-) (pilA) [Piscirickettsia salmonis strain Ps-8079A]
MEKNNANSNSEKKQGLFARLKSGLSKTRENLASGITTLLIGEKKIDDELLEDLETQLLMADVGIDATTHIITDLTKRLDR
NELTDTQKAMEALKENLSGLLDPCNIPLEIPETKDPFVILVVGVNGAGKTTTIGKLAKYYQTQGKSVMLAAGDTFRAAAV
EQLQVWGERNDIPVVAQHTGADSASVIFDAHNSAKAHGYDILIADTAGRLHTQSNLMEELKKVKRVLGKVDNTAPHEVLL
VLDSGTGQNAINQAEQFHQAVNVSGIVMTKLDGTAKGGVIFAIAKKMKLPIRYIGVGEKIDDLRPFKSDEFIEALFG

Nucleotide


Download         Length: 954 bp        

>NTDB_id=419756 GW535_RS19030 WP_210428201.1 298366..299319(-) (pilA) [Piscirickettsia salmonis strain Ps-8079A]
ATTGAAAAAAACAACGCCAATAGTAATAGTGAGAAAAAGCAAGGCTTATTCGCCCGCTTAAAATCAGGCCTCAGTAAAAC
CCGTGAAAATCTAGCCTCAGGCATTACCACACTTTTAATCGGCGAGAAAAAAATCGATGATGAGCTGCTGGAAGATTTAG
AAACTCAGCTACTCATGGCAGATGTTGGCATTGATGCAACGACTCATATCATTACTGACTTAACCAAGCGTCTTGATCGC
AACGAACTTACAGATACCCAAAAAGCGATGGAAGCGCTCAAAGAAAATTTATCTGGGCTGCTCGATCCATGCAATATTCC
CCTAGAAATTCCAGAAACAAAAGATCCCTTTGTGATTTTAGTCGTCGGCGTGAATGGCGCAGGGAAGACCACAACCATTG
GTAAACTTGCCAAATATTATCAAACCCAGGGTAAAAGCGTGATGCTTGCTGCTGGTGATACCTTCCGTGCCGCCGCGGTT
GAACAATTACAAGTCTGGGGAGAACGTAACGATATTCCGGTAGTTGCCCAGCATACCGGTGCTGATAGCGCATCAGTAAT
TTTCGATGCGCATAACTCAGCCAAAGCACATGGTTATGACATTCTAATTGCCGATACCGCAGGCCGACTGCATACACAAA
GCAACCTGATGGAAGAACTAAAAAAGGTAAAACGTGTGCTTGGCAAAGTCGACAATACAGCACCGCATGAAGTTTTACTA
GTCCTAGACTCAGGCACTGGACAAAACGCTATTAACCAAGCTGAGCAGTTTCATCAGGCTGTCAATGTCTCAGGCATTGT
CATGACTAAACTCGATGGCACGGCTAAAGGTGGGGTCATTTTTGCTATCGCTAAGAAAATGAAGTTACCTATCCGTTATA
TTGGCGTCGGCGAAAAAATTGATGATCTACGCCCTTTTAAATCAGATGAATTTATTGAGGCTTTATTTGGCTAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(116-316)

SRP54-type protein, helical bundle domain (SRP54_N)

(33-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

54.693

97.476

0.533

Multiple sequence alignment