Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GSF17_RS02245 Genome accession   NZ_CP048051
Coordinates   498595..499647 (+) Length   350 a.a.
NCBI ID   WP_162094294.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain zm-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 493595..504647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSF17_RS02235 - 494559..495827 (+) 1269 WP_162094292.1 malic enzyme-like NAD(P)-binding protein -
  GSF17_RS02240 - 495943..498378 (-) 2436 WP_202928212.1 penicillin-binding protein 1A -
  GSF17_RS02245 pilM 498595..499647 (+) 1053 WP_162094294.1 pilus assembly protein PilM Machinery gene
  GSF17_RS02250 - 499647..500213 (+) 567 WP_038582728.1 PilN domain-containing protein -
  GSF17_RS02255 - 500210..500827 (+) 618 WP_162094295.1 type 4a pilus biogenesis protein PilO -
  GSF17_RS02260 - 500824..501351 (+) 528 WP_162094296.1 pilus assembly protein PilP -
  GSF17_RS02265 pilQ 501365..503437 (+) 2073 WP_162094297.1 type IV pilus secretin PilQ Machinery gene
  GSF17_RS02270 aroK 503442..503960 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37051.50 Da        Isoelectric Point: 4.9966

>NTDB_id=419690 GSF17_RS02245 WP_162094294.1 498595..499647(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain zm-1]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALVAGASVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSKEDAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVSGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=419690 GSF17_RS02245 WP_162094294.1 498595..499647(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain zm-1]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCCTGTCTCGTCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTTTGCGATGACGATCGGGAAAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGATGAGGCTGCGATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTATTGGCGGCCTGTCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCACGGGTGGTGGACCTGGAAGTCTGCGCGCTGGAACGCTCGTTCGCCCTGGTGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCATATCCAGCGTCGTTACGGGCTGTCGAAAGAAGACGCTGGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGACTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTTCGGGACTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCCGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTTGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.83

100

0.551
  comM Acinetobacter nosocomialis M2

54.545

100

0.549
  comM Acinetobacter baylyi ADP1

52.273

100

0.526
  pilM Legionella pneumophila strain ERS1305867

45.198

100

0.457

Multiple sequence alignment