Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GWK28_RS01475 Genome accession   NZ_CP047973
Coordinates   315292..316032 (+) Length   246 a.a.
NCBI ID   WP_187142584.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain DETAB-P2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 310292..321032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWK28_RS01460 (GWK28_01460) ponA 310877..313432 (-) 2556 WP_187142583.1 penicillin-binding protein PBP1a -
  GWK28_RS01465 (GWK28_01465) pilM 313596..314654 (+) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  GWK28_RS01470 (GWK28_01470) pilN 314654..315295 (+) 642 WP_000201224.1 PilN domain-containing protein Machinery gene
  GWK28_RS01475 (GWK28_01475) pilO 315292..316032 (+) 741 WP_187142584.1 type 4a pilus biogenesis protein PilO Machinery gene
  GWK28_RS01480 (GWK28_01480) pilP 316043..316570 (+) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  GWK28_RS01485 (GWK28_01485) pilQ 316633..318798 (+) 2166 WP_002062175.1 type IV pilus secretin PilQ family protein Machinery gene
  GWK28_RS01490 (GWK28_01490) aroK 318810..319379 (+) 570 WP_000643712.1 shikimate kinase AroK -
  GWK28_RS01495 (GWK28_01495) aroB 319408..320490 (+) 1083 WP_001193997.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27791.57 Da        Isoelectric Point: 4.9608

>NTDB_id=418889 GWK28_RS01475 WP_187142584.1 315292..316032(+) (pilO) [Acinetobacter baumannii strain DETAB-P2]
MSQNELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDISLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=418889 GWK28_RS01475 WP_187142584.1 315292..316032(+) (pilO) [Acinetobacter baumannii strain DETAB-P2]
ATGAGTCAAAACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAGCTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

99.187

100

0.992
  comO Acinetobacter baylyi ADP1

68.595

98.374

0.675

Multiple sequence alignment