Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   E3T16_RS02400 Genome accession   NZ_CP047777
Coordinates   446621..447118 (+) Length   165 a.a.
NCBI ID   WP_029650985.1    Uniprot ID   -
Organism   Staphylococcus aureus strain UP_1632     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 441621..452118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3T16_RS02360 (E3T16_02360) - 441665..442651 (+) 987 WP_000161311.1 ROK family glucokinase -
  E3T16_RS02365 (E3T16_02365) - 442651..442980 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E3T16_RS02370 (E3T16_02370) - 442977..443600 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  E3T16_RS02375 (E3T16_02375) comGA 443652..444626 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  E3T16_RS02380 (E3T16_02380) comGB 444598..445668 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  E3T16_RS02385 (E3T16_02385) comGC 445682..445993 (+) 312 WP_160177506.1 competence type IV pilus major pilin ComGC Machinery gene
  E3T16_RS02390 (E3T16_02390) comGD 445971..446417 (+) 447 WP_160177508.1 competence type IV pilus minor pilin ComGD Machinery gene
  E3T16_RS02395 (E3T16_02395) comGE 446404..446703 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  E3T16_RS02400 (E3T16_02400) comGF 446621..447118 (+) 498 WP_029650985.1 competence type IV pilus minor pilin ComGF Machinery gene
  E3T16_RS02405 (E3T16_02405) - 447215..447361 (+) 147 WP_001792109.1 hypothetical protein -
  E3T16_RS02410 (E3T16_02410) - 447351..447875 (+) 525 WP_001015120.1 shikimate kinase -
  E3T16_RS02415 (E3T16_02415) gcvT 448034..449125 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  E3T16_RS02420 (E3T16_02420) gcvPA 449145..450491 (+) 1347 WP_160177510.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  E3T16_RS02425 (E3T16_02425) gcvPB 450484..451956 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.05 Da        Isoelectric Point: 10.3787

>NTDB_id=415750 E3T16_RS02400 WP_029650985.1 446621..447118(+) (comGF) [Staphylococcus aureus strain UP_1632]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEKMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=415750 E3T16_RS02400 WP_029650985.1 446621..447118(+) (comGF) [Staphylococcus aureus strain UP_1632]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAAAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

99.394

100

0.994
  comGF Staphylococcus aureus MW2

95.758

100

0.958

Multiple sequence alignment