Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   HUG14_RS03325 Genome accession   NZ_CP060580
Coordinates   676681..677682 (-) Length   333 a.a.
NCBI ID   WP_003131994.1    Uniprot ID   Q9CDL3
Organism   Lactococcus lactis strain K_LL005     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 671681..682682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUG14_RS03290 (HUG14_03290) - 671969..672334 (-) 366 WP_003131985.1 MerR family transcriptional regulator -
  HUG14_RS03295 (HUG14_03295) - 672435..672872 (-) 438 WP_058219793.1 low molecular weight protein-tyrosine-phosphatase -
  HUG14_RS03300 (HUG14_03300) - 673016..673180 (-) 165 WP_003131988.1 hypothetical protein -
  HUG14_RS03305 (HUG14_03305) - 673285..673902 (-) 618 WP_012898695.1 AAA family ATPase -
  HUG14_RS03310 (HUG14_03310) - 673899..675083 (-) 1185 WP_014570851.1 ABC transporter permease -
  HUG14_RS03315 (HUG14_03315) - 675150..675434 (-) 285 WP_233610988.1 hypothetical protein -
  HUG14_RS03320 (HUG14_03320) - 675660..676544 (-) 885 WP_058203823.1 XRE/MutR family transcriptional regulator -
  HUG14_RS03325 (HUG14_03325) ruvB 676681..677682 (-) 1002 WP_003131994.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  HUG14_RS03330 (HUG14_03330) ruvA 677809..678402 (-) 594 WP_003131995.1 Holliday junction branch migration protein RuvA Machinery gene
  HUG14_RS03335 (HUG14_03335) hexB 678512..680482 (-) 1971 WP_058223262.1 DNA mismatch repair endonuclease MutL Machinery gene
  HUG14_RS03340 (HUG14_03340) - 680611..681321 (-) 711 WP_033899281.1 hypothetical protein -

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 37633.17 Da        Isoelectric Point: 4.5825

>NTDB_id=415326 HUG14_RS03325 WP_003131994.1 676681..677682(-) (ruvB) [Lactococcus lactis strain K_LL005]
MNDILNKEPMEEDYGIEKSLRPQFFNQYIGQDKVKEQLEIFIKAAKMREEVLDHVLLFGPPGLGKTTMAFVIANELGVNI
KQTAGPAIEKPGDLVAILNELEPGDVLFIDEIHRMPMQVEEVLYSAMEDFYIDIMLGSGDGSRSVHLDLPPFTLVGATTR
AGMLSNPLRARFGISSHMEYYQERDLEEIVKRTADIFEVEVIDNAALEIALRSRGTPRIANRLLKRVRDFAQIMGDGRVD
KAITDKALSILDVDAAGLDYIDQKILRTMIEMYHGGPVGIGTLAVNIAEDRETVEDMYEPYLIQKGFLMRTKQGRKVTQR
AYEHLGYVYNEED

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=415326 HUG14_RS03325 WP_003131994.1 676681..677682(-) (ruvB) [Lactococcus lactis strain K_LL005]
ATGAACGATATATTAAACAAAGAGCCAATGGAAGAAGATTACGGTATTGAAAAATCTCTACGTCCGCAATTTTTTAATCA
ATATATTGGTCAAGATAAAGTCAAAGAACAATTAGAAATCTTTATTAAAGCAGCAAAAATGCGTGAAGAAGTTTTGGACC
ATGTATTACTTTTTGGTCCTCCGGGACTTGGTAAGACAACAATGGCATTTGTCATTGCCAATGAGTTGGGAGTTAATATT
AAACAAACAGCGGGTCCTGCCATTGAAAAGCCAGGAGATTTAGTTGCGATTTTGAATGAATTGGAACCTGGCGATGTGCT
ATTTATTGATGAAATTCATCGGATGCCAATGCAAGTTGAAGAAGTTCTTTATAGCGCTATGGAAGATTTCTACATTGATA
TTATGCTTGGTTCTGGTGATGGTTCACGCTCGGTTCATTTAGATTTGCCGCCATTTACTCTAGTTGGGGCAACAACAAGA
GCTGGAATGCTTTCGAATCCATTGCGTGCCCGTTTTGGAATTTCAAGTCATATGGAGTATTATCAAGAGCGTGATTTAGA
AGAAATTGTTAAAAGAACAGCTGATATTTTTGAAGTCGAAGTTATTGATAATGCCGCTTTAGAGATTGCTTTACGTAGTC
GAGGGACTCCACGGATTGCCAATCGTTTACTTAAACGAGTGCGTGATTTTGCTCAAATAATGGGGGATGGCCGAGTGGAT
AAAGCAATTACCGATAAGGCCTTGTCTATTCTTGATGTTGATGCCGCTGGTTTGGACTATATTGACCAAAAAATCCTCCG
CACAATGATTGAAATGTACCACGGTGGTCCTGTTGGGATTGGAACTTTGGCGGTAAATATTGCCGAAGACCGTGAAACGG
TCGAGGATATGTATGAACCTTATTTGATTCAAAAAGGATTTTTGATGAGAACTAAACAGGGACGTAAGGTGACCCAAAGA
GCCTATGAACACCTAGGTTATGTATACAATGAAGAAGATTGA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(256-325)

RuvB AAA lid domain (AAA_lid_4)

(181-254)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(20-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CDL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Streptococcus pneumoniae R6

78.614

99.7

0.784
  ruvB Streptococcus pneumoniae D39

78.614

99.7

0.784
  ruvB Streptococcus pneumoniae TIGR4

78.313

99.7

0.781
  ruvB Bacillus subtilis subsp. subtilis str. 168

61.491

96.697

0.595
  ruvB Helicobacter pylori 26695

51.242

96.697

0.495
  ruvB Synechocystis sp. PCC 6803

51.613

93.093

0.48