Detailed information NTG0415264

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H4B46_RS10320
• Genome accession: NZ_CP060504
• Coordinates: 2159270..2160388 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A086HYC5
• Organism: Acinetobacter baumannii strain AbCTX9
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2154270..2165388

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H4B46_RS10290 (H4B46_10275)	-	2155099..2155947 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
H4B46_RS10295 (H4B46_10280)	-	2156200..2156655 (+)	456	WP_000782976.1	bacteriohemerythrin	-
H4B46_RS10300 (H4B46_10285)	-	2156808..2157875 (+)	1068	WP_029776981.1	membrane protein	-
H4B46_RS10305 (H4B46_10290)	-	2157875..2158198 (+)	324	WP_000442585.1	RnfH family protein	-
H4B46_RS10310 (H4B46_10295)	bamE	2158253..2158651 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
H4B46_RS10315 (H4B46_10300)	fur	2158763..2159200 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
H4B46_RS10320 (H4B46_10305)	pilU	2159270..2160388 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H4B46_RS10325 (H4B46_10310)	pilT	2160416..2161453 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
H4B46_RS10330 (H4B46_10315)	-	2161580..2162272 (+)	693	WP_024432389.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=415264 H4B46_RS10320 WP_000347036.1 2159270..2160388(-) (pilU) [Acinetobacter baumannii strain AbCTX9]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=415264 H4B46_RS10320 WP_000347036.1 2159270..2160388(-) (pilU) [Acinetobacter baumannii strain AbCTX9]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A086HYC5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392