Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GNE05_RS12980 Genome accession   NZ_CP047644
Coordinates   2653058..2654128 (-) Length   356 a.a.
NCBI ID   WP_014418378.1    Uniprot ID   A0AAP4DGV9
Organism   Bacillus sp. AM1(2019)     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2648058..2659128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNE05_RS12930 (GNE05_12930) - 2648117..2648701 (-) 585 WP_022552967.1 signal peptidase I -
  GNE05_RS12935 (GNE05_12935) tapA 2648673..2649344 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  GNE05_RS12940 (GNE05_12940) - 2649603..2649932 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  GNE05_RS12945 (GNE05_12945) - 2649973..2650152 (-) 180 WP_003153093.1 YqzE family protein -
  GNE05_RS12950 (GNE05_12950) comGG 2650209..2650586 (-) 378 WP_061862389.1 competence type IV pilus minor pilin ComGG Machinery gene
  GNE05_RS12955 (GNE05_12955) comGF 2650587..2650982 (-) 396 WP_061862390.1 competence type IV pilus minor pilin ComGF -
  GNE05_RS12960 (GNE05_12960) comGE 2650996..2651310 (-) 315 WP_017418140.1 competence type IV pilus minor pilin ComGE Machinery gene
  GNE05_RS12965 (GNE05_12965) comGD 2651294..2651731 (-) 438 WP_095061019.1 competence type IV pilus minor pilin ComGD Machinery gene
  GNE05_RS12970 (GNE05_12970) comGC 2651721..2652029 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  GNE05_RS12975 (GNE05_12975) comGB 2652034..2653071 (-) 1038 WP_014418377.1 competence type IV pilus assembly protein ComGB Machinery gene
  GNE05_RS12980 (GNE05_12980) comGA 2653058..2654128 (-) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  GNE05_RS12985 (GNE05_12985) - 2654321..2655271 (-) 951 WP_160182873.1 magnesium transporter CorA family protein -
  GNE05_RS12990 (GNE05_12990) - 2655417..2656718 (+) 1302 WP_029973873.1 hemolysin family protein -
  GNE05_RS12995 (GNE05_12995) - 2656756..2657586 (-) 831 WP_021494316.1 STAS domain-containing protein -
  GNE05_RS13005 (GNE05_13005) - 2657792..2658172 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  GNE05_RS13010 (GNE05_13010) - 2658409..2658654 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.81 Da        Isoelectric Point: 9.3485

>NTDB_id=414883 GNE05_RS12980 WP_014418378.1 2653058..2654128(-) (comGA) [Bacillus sp. AM1(2019)]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=414883 GNE05_RS12980 WP_014418378.1 2653058..2654128(-) (comGA) [Bacillus sp. AM1(2019)]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGATCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGTGCCGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment