Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GRX76_RS08270 Genome accession   NZ_CP047569
Coordinates   2019930..2021111 (+) Length   393 a.a.
NCBI ID   WP_160152873.1    Uniprot ID   -
Organism   Microbulbifer sp. ALW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2021783..2057479 2019930..2021111 flank 672


Gene organization within MGE regions


Location: 2019930..2057479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRX76_RS08270 pilU 2019930..2021111 (+) 1182 WP_160152873.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GRX76_RS08275 - 2021780..2022628 (+) 849 WP_236250604.1 glycosyltransferase family 2 protein -
  GRX76_RS08280 - 2022659..2023144 (+) 486 WP_160152875.1 UDP-N-acetylglucosamine--LPS N-acetylglucosamine transferase -
  GRX76_RS08285 - 2023274..2023756 (+) 483 WP_160152876.1 glycosyltransferase -
  GRX76_RS08290 - 2023817..2025127 (+) 1311 WP_160152877.1 O-antigen ligase -
  GRX76_RS19435 - 2025114..2025563 (+) 450 WP_160152878.1 DapH/DapD/GlmU-related protein -
  GRX76_RS08300 - 2025547..2026527 (+) 981 WP_160152879.1 glycosyltransferase family 2 protein -
  GRX76_RS08305 - 2026524..2027645 (+) 1122 WP_160152880.1 acyltransferase -
  GRX76_RS08310 - 2027629..2028393 (+) 765 WP_160152881.1 SGNH/GDSL hydrolase family protein -
  GRX76_RS08315 - 2028402..2029370 (-) 969 WP_160152882.1 glycosyltransferase family A protein -
  GRX76_RS08320 - 2029367..2030854 (-) 1488 WP_160152883.1 lipopolysaccharide biosynthesis protein -
  GRX76_RS08325 - 2030925..2031995 (-) 1071 WP_160152884.1 glycosyltransferase -
  GRX76_RS08330 - 2032171..2033118 (-) 948 WP_160152885.1 sulfotransferase -
  GRX76_RS08335 - 2033102..2034001 (-) 900 WP_160152886.1 hypothetical protein -
  GRX76_RS08340 - 2034036..2041247 (-) 7212 WP_160152887.1 Ig-like domain-containing protein -
  GRX76_RS08345 - 2041791..2044160 (-) 2370 WP_160152888.1 discoidin domain-containing protein -
  GRX76_RS08350 - 2044290..2045120 (-) 831 WP_160152889.1 CpsD/CapB family tyrosine-protein kinase -
  GRX76_RS08355 - 2045129..2046718 (-) 1590 WP_160152890.1 lipopolysaccharide biosynthesis protein -
  GRX76_RS08360 - 2046724..2047881 (-) 1158 WP_160152891.1 hypothetical protein -
  GRX76_RS08365 - 2047883..2048461 (-) 579 WP_160152892.1 polysaccharide biosynthesis/export family protein -
  GRX76_RS08370 - 2049476..2050321 (+) 846 WP_160152893.1 helix-turn-helix transcriptional regulator -
  GRX76_RS08375 - 2050321..2051751 (+) 1431 WP_160152894.1 undecaprenyl-phosphate glucose phosphotransferase -
  GRX76_RS08380 - 2051984..2052445 (+) 462 WP_160152895.1 hypothetical protein -
  GRX76_RS08385 xrtD 2052642..2054195 (+) 1554 WP_160152896.1 VPLPA-CTERM-specific exosortase XrtD -
  GRX76_RS08390 - 2054195..2056570 (+) 2376 WP_160152897.1 tetratricopeptide repeat protein -
  GRX76_RS08395 - 2056580..2057479 (+) 900 WP_160152898.1 ExeA family protein -

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 43928.36 Da        Isoelectric Point: 5.9939

>NTDB_id=414218 GRX76_RS08270 WP_160152873.1 2019930..2021111(+) (pilU) [Microbulbifer sp. ALW1]
MDFTGYLRVLAKNDGSDLYLSTGAPPCAKFQGVLKPLSKEPLAPGEIEKIATEIMDEEQREEFKRELEMNLAMTIPHVGR
FRINIFWQRNQVSIVARNIVTEIPKFDDLKLPPILKDVVMAKRGLVLFVGGTGSGKSTSLAALIDHRNTNSGGHIITIED
PVEYVHRHKKSVINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLAISTLHANNANQALDRI
VNFFPEERRPQLLMSLSQNIRAFVSQRLVPTVDGKRCAAVEILLGTQTINELILKGEFHNIKEMMEKSENLGMQTFDAAL
FKLYVEERISHEEAIKNADSANNLRLRIKMYEEGKKPKAVPEEDEAKSVGAIAGGGLALSLESIDEEEEVVNF

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=414218 GRX76_RS08270 WP_160152873.1 2019930..2021111(+) (pilU) [Microbulbifer sp. ALW1]
ATGGATTTCACCGGTTATCTCCGTGTACTCGCCAAAAACGATGGCTCTGACCTTTACCTCAGTACCGGAGCACCGCCCTG
TGCCAAGTTTCAGGGTGTACTGAAGCCCCTGAGCAAGGAGCCACTTGCGCCCGGGGAGATCGAGAAGATCGCCACAGAAA
TCATGGATGAGGAGCAGCGCGAGGAGTTCAAGCGCGAACTGGAAATGAACCTGGCGATGACCATTCCCCATGTGGGGCGT
TTCCGGATCAATATCTTCTGGCAGCGCAACCAGGTTTCCATTGTTGCGCGAAATATCGTCACCGAAATCCCGAAGTTTGA
TGACCTGAAGTTACCGCCCATCCTCAAGGATGTGGTGATGGCCAAGCGTGGCCTGGTGCTGTTTGTCGGTGGCACCGGTT
CCGGAAAATCCACCTCTCTGGCAGCGCTGATCGACCATCGCAACACCAACAGTGGTGGACACATCATCACCATTGAGGAT
CCCGTGGAGTATGTGCACCGGCACAAGAAGAGTGTGATCAACCAGCGGGAAGTCGGTGTCGATACCCGCAGCTTTCACGC
CGCCCTGAAAAATACCCTGCGCCAGGCGCCAGACGTAATTCTGATTGGCGAGATCCGCGATCGCGAAACCATGGAGCACG
CCATCGCCTTTGCCGAGACCGGCCACCTGGCGATCTCCACCCTTCACGCCAACAACGCCAACCAGGCCCTCGACCGGATT
GTGAATTTCTTCCCCGAAGAGCGCCGCCCACAACTGCTGATGTCCCTGTCGCAGAATATCCGCGCTTTTGTTTCACAGCG
CCTGGTGCCAACGGTCGATGGCAAGCGCTGTGCCGCGGTGGAAATCCTGCTGGGTACCCAGACGATTAACGAGCTGATCC
TCAAGGGTGAATTCCACAACATCAAGGAAATGATGGAGAAGTCGGAAAACCTTGGCATGCAGACGTTTGACGCCGCACTG
TTCAAACTCTACGTGGAAGAGCGGATCAGCCACGAGGAGGCGATCAAGAACGCGGACTCTGCCAATAACCTGCGTCTGCG
TATCAAAATGTATGAAGAGGGAAAGAAACCTAAGGCCGTGCCTGAGGAGGATGAGGCGAAAAGCGTTGGCGCCATTGCCG
GCGGTGGGCTCGCGCTGTCCCTTGAGTCCATTGATGAAGAGGAAGAAGTGGTCAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.367

90.076

0.499
  pilU Acinetobacter baylyi ADP1

53.76

91.349

0.491
  pilU Vibrio cholerae strain A1552

48.857

89.059

0.435
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.068

86.26

0.372

Multiple sequence alignment