Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GRX76_RS01580 Genome accession   NZ_CP047569
Coordinates   414581..415657 (-) Length   358 a.a.
NCBI ID   WP_160151694.1    Uniprot ID   -
Organism   Microbulbifer sp. ALW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 409581..420657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRX76_RS01555 aroK 410087..410608 (-) 522 WP_201276954.1 shikimate kinase AroK -
  GRX76_RS01560 pilQ 410608..412845 (-) 2238 WP_160151690.1 type IV pilus secretin PilQ family protein -
  GRX76_RS01565 - 412849..413376 (-) 528 WP_160151691.1 pilus assembly protein PilP -
  GRX76_RS01570 - 413373..413993 (-) 621 WP_160151692.1 type 4a pilus biogenesis protein PilO -
  GRX76_RS01575 - 413996..414577 (-) 582 WP_160151693.1 PilN domain-containing protein -
  GRX76_RS01580 pilM 414581..415657 (-) 1077 WP_160151694.1 pilus assembly protein PilM Machinery gene
  GRX76_RS01585 - 415813..418515 (+) 2703 WP_160151695.1 penicillin-binding protein 1A -
  GRX76_RS01590 - 418676..419161 (-) 486 WP_160151696.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39039.36 Da        Isoelectric Point: 4.3560

>NTDB_id=414206 GRX76_RS01580 WP_160151694.1 414581..415657(-) (pilM) [Microbulbifer sp. ALW1]
MGMLPFLNKGPKAMLGLDISSTSVKLLELSRSGDKYRVESYATEPLPPNAVVDKNINDVGAIAEAIRKVVRRSKTRLRQA
AVAVSGSAVITKTLDMPADLSDDALEAQIALEADQYIPYPLDEVNLDFEVQGPSEKGEGQVEVLLAACRSENVEQRISAL
TEADMQPGVVDVEAYAIERAYTLMDNQFPAQEQLVVAVIDIGATMSALSVMVDGRTIYTREQLFGGRQLTDEIQRRYGLS
AEEATLAKRQGGSGLPDDYYPEVLEPFRDAVVQQVTRSLQFFYSSTSYSDVDYILLGGGVAAMEGLADLVGQKLNKPTMV
ANPFRDMSVASRVNRQALVNEAPSLMIAAGLAMREREF

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=414206 GRX76_RS01580 WP_160151694.1 414581..415657(-) (pilM) [Microbulbifer sp. ALW1]
ATGGGGATGCTCCCTTTCTTGAATAAGGGCCCGAAAGCCATGCTTGGGCTCGATATTAGCTCCACCTCGGTAAAGTTGCT
TGAACTGAGTCGCAGCGGTGACAAATATCGCGTGGAAAGCTATGCAACCGAGCCTCTGCCACCGAATGCGGTTGTGGATA
AGAATATCAATGACGTTGGTGCGATCGCCGAGGCCATCCGCAAGGTGGTGCGCCGCTCTAAAACCCGCCTGCGTCAAGCA
GCCGTGGCGGTTTCCGGTTCTGCGGTTATCACCAAGACCCTGGATATGCCGGCAGACCTCTCTGACGACGCCCTGGAAGC
GCAGATAGCGCTGGAGGCCGACCAATACATTCCCTACCCGCTGGACGAGGTGAATCTCGACTTCGAGGTGCAGGGACCTT
CCGAGAAGGGCGAGGGGCAGGTTGAGGTGTTGCTGGCGGCCTGTCGTAGCGAGAATGTGGAACAGCGCATCTCAGCCCTC
ACCGAAGCGGATATGCAGCCTGGGGTTGTGGACGTCGAAGCCTACGCCATCGAGCGCGCTTATACCTTGATGGACAACCA
GTTTCCCGCGCAGGAGCAGCTGGTAGTGGCGGTCATCGATATCGGCGCCACCATGAGTGCGCTGTCGGTGATGGTGGACG
GACGCACCATTTACACCCGTGAGCAGCTGTTTGGTGGGCGCCAGCTTACTGACGAAATCCAGCGTCGCTACGGTCTGTCG
GCGGAAGAGGCGACGCTGGCCAAGCGCCAGGGCGGCAGCGGTCTACCAGACGATTATTACCCCGAGGTACTGGAGCCCTT
CCGCGATGCGGTGGTGCAGCAGGTGACTCGTTCCCTGCAGTTCTTCTACTCCTCGACTTCCTATAGCGATGTGGATTACA
TCCTGCTCGGTGGCGGTGTCGCCGCCATGGAAGGGCTTGCCGACCTGGTGGGCCAGAAACTCAACAAACCCACAATGGTG
GCTAACCCCTTCCGGGACATGAGCGTGGCATCCCGTGTCAATCGCCAGGCCCTGGTTAACGAGGCGCCGTCGCTGATGAT
TGCCGCCGGCCTAGCCATGCGCGAGCGGGAGTTCTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

52.557

98.324

0.517
  comM Acinetobacter nosocomialis M2

52.557

98.324

0.517
  comM Acinetobacter baylyi ADP1

48.295

98.324

0.475
  pilM Legionella pneumophila strain ERS1305867

44.382

99.441

0.441

Multiple sequence alignment