Detailed information NTG0413660

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I5102_RS02595
• Genome accession: NZ_CP060240
• Coordinates: 556161..557309 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain G-I-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 551161..562309

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I5102_RS02570 (I5102_000530)	-	552312..552608 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
I5102_RS02575 (I5102_000531)	-	552605..553198 (-)	594	WP_003084544.1	YggT family protein	-
I5102_RS02580 (I5102_000532)	proC	553209..554030 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
I5102_RS02585 (I5102_000533)	-	554042..554734 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
I5102_RS02590 (I5102_000534)	pilT	554949..555983 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
I5102_RS02595 (I5102_000535)	pilU	556161..557309 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
I5102_RS02600 (I5102_000536)	czcD	557317..558216 (-)	900	WP_196331883.1	CDF family zinc transporter CzcD	-
I5102_RS02605 (I5102_000537)	-	558370..558774 (+)	405	WP_003084560.1	NINE protein	-
I5102_RS02610 (I5102_000538)	-	559017..560390 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
I5102_RS02615 (I5102_000539)	-	560387..561571 (+)	1185	WP_196331884.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=413660 I5102_RS02595 WP_003100959.1 556161..557309(+) (pilU) [Pseudomonas aeruginosa strain G-I-1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=413660 I5102_RS02595 WP_003100959.1 556161..557309(+) (pilU) [Pseudomonas aeruginosa strain G-I-1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382