Detailed information NTG0413276

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H5409_RS02130
• Genome accession: NZ_CP060086
• Coordinates: 461116..462264 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain JNQH-PA57
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 456116..467264

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H5409_RS02105 (H5409_02105)	-	457267..457563 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
H5409_RS02110 (H5409_02110)	-	457560..458153 (-)	594	WP_003084544.1	YggT family protein	-
H5409_RS02115 (H5409_02115)	proC	458164..458985 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
H5409_RS02120 (H5409_02120)	-	458997..459689 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
H5409_RS02125 (H5409_02125)	pilT	459904..460938 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
H5409_RS02130 (H5409_02130)	pilU	461116..462264 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
H5409_RS02135 (H5409_02135)	czcD	462272..463171 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
H5409_RS02140 (H5409_02140)	-	463326..463730 (+)	405	WP_003084560.1	NINE protein	-
H5409_RS02145 (H5409_02145)	-	463973..465346 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
H5409_RS02150 (H5409_02150)	-	465343..466527 (+)	1185	WP_023092310.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=413276 H5409_RS02130 WP_003110016.1 461116..462264(+) (pilU) [Pseudomonas aeruginosa strain JNQH-PA57]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=413276 H5409_RS02130 WP_003110016.1 461116..462264(+) (pilU) [Pseudomonas aeruginosa strain JNQH-PA57]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382