Detailed information NTG0412458

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H5023_RS28610
• Genome accession: NZ_CP060005
• Coordinates: 6084130..6085278 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain WK190
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6079130..6090278

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H5023_RS28590 (H5023_28485)	-	6079868..6081052 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
H5023_RS28595 (H5023_28490)	-	6081049..6082422 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
H5023_RS28600 (H5023_28495)	-	6082665..6083069 (-)	405	WP_003084560.1	NINE protein	-
H5023_RS28605 (H5023_28500)	czcD	6083223..6084122 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
H5023_RS28610 (H5023_28505)	pilU	6084130..6085278 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
H5023_RS28615 (H5023_28510)	pilT	6085456..6086490 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
H5023_RS28620 (H5023_28515)	-	6086705..6087397 (+)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
H5023_RS28625 (H5023_28520)	proC	6087409..6088230 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
H5023_RS28630 (H5023_28525)	-	6088241..6088834 (+)	594	WP_003084544.1	YggT family protein	-
H5023_RS28635 (H5023_28530)	-	6088831..6089127 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=412458 H5023_RS28610 WP_003100959.1 6084130..6085278(-) (pilU) [Pseudomonas aeruginosa strain WK190]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=412458 H5023_RS28610 WP_003100959.1 6084130..6085278(-) (pilU) [Pseudomonas aeruginosa strain WK190]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382