Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   N015_RS22420 Genome accession   NZ_CP047265
Coordinates   5031634..5032974 (+) Length   446 a.a.
NCBI ID   WP_024685591.1    Uniprot ID   -
Organism   Pseudomonas asturiensis strain CC1524     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5026634..5037974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N015_RS22395 (N015_22395) pgeF 5026830..5027558 (-) 729 WP_024685588.1 peptidoglycan editing factor PgeF -
  N015_RS22400 (N015_22400) rluD 5027555..5028517 (-) 963 WP_024685589.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  N015_RS22405 (N015_22405) - 5028667..5029689 (+) 1023 WP_024685590.1 outer membrane protein assembly factor BamD -
  N015_RS22410 (N015_22410) - 5029810..5030049 (+) 240 WP_080265980.1 PP0621 family protein -
  N015_RS22415 (N015_22415) pilS 5030039..5031625 (+) 1587 WP_032622576.1 sensor histidine kinase Regulator
  N015_RS22420 (N015_22420) pilR 5031634..5032974 (+) 1341 WP_024685591.1 sigma-54-dependent transcriptional regulator Regulator
  N015_RS22425 (N015_22425) - 5033171..5033386 (-) 216 Protein_4442 NAD(P)/FAD-dependent oxidoreductase -
  N015_RS22430 (N015_22430) - 5033618..5033836 (-) 219 Protein_4443 NAD(P)/FAD-dependent oxidoreductase -
  N015_RS22435 (N015_22435) - 5034065..5034280 (-) 216 Protein_4444 NAD(P)/FAD-dependent oxidoreductase -
  N015_RS22440 (N015_22440) thiO 5034508..5035620 (-) 1113 WP_159372212.1 glycine oxidase ThiO -
  N015_RS22445 (N015_22445) - 5035647..5036048 (-) 402 WP_024688922.1 type IV pilin protein -

Sequence

Protein


Download         Length: 446 a.a.        Molecular weight: 49915.16 Da        Isoelectric Point: 6.1359

>NTDB_id=411930 N015_RS22420 WP_024685591.1 5031634..5032974(+) (pilR) [Pseudomonas asturiensis strain CC1524]
MTPRQKILIVDDEPDIRELLEITLGRMKLDTRSACNVAEARDYLAREPFELCLTDMRLPDGNGLELVQHIQQNFAHVPVA
MITAHGSLDTAIQALKAGAFDFLTKPVDLGRLRELVNSALRLTPIAQPVRALDNRLLGDSPPMRTLRNQITKLARSQAPV
YISGESGSGKELVARLIHEQGPRGEKPFVPVNCGAIPTDLMESEFFGHRKGSFTGAHEDKPGLFQAAQNGTLFLDEVADL
PLAMQVKLLRAIQEKSIRSVGGQQEQVVDVRILCATHKDLSVEVAAGRFRQDLYYRLNVIELCVPSLRERREDIDQLAAI
VLQRLAANSGLPAARLNAQALETLKNYRFPGNVRELENMLERAYTLCENDEIHTSDLRLAESVRTAESDGPNLADIDNLE
DYLEGIERKLILQALEETRWNRTAAAQRLSLSFRSMRYRLKKLGLD

Nucleotide


Download         Length: 1341 bp        

>NTDB_id=411930 N015_RS22420 WP_024685591.1 5031634..5032974(+) (pilR) [Pseudomonas asturiensis strain CC1524]
ATGACCCCACGGCAAAAGATCCTGATCGTTGACGATGAGCCGGACATTCGCGAACTTCTGGAGATTACTCTGGGCCGAAT
GAAACTCGACACCCGCAGCGCCTGCAATGTTGCCGAAGCACGTGATTACCTGGCTCGCGAACCGTTCGAACTGTGCCTGA
CGGACATGCGCCTGCCCGACGGCAATGGCCTGGAGCTGGTGCAGCATATCCAGCAGAATTTTGCGCACGTTCCCGTTGCG
ATGATCACGGCGCATGGCAGCCTGGACACCGCCATTCAGGCGCTCAAGGCCGGTGCGTTCGACTTCCTGACCAAGCCGGT
GGATCTGGGCAGGCTGCGCGAACTGGTCAACAGTGCGTTACGCCTCACGCCCATCGCCCAGCCGGTGCGCGCCCTGGATA
ACCGCCTGTTGGGTGACTCGCCGCCCATGCGTACGCTGCGCAACCAGATCACCAAGCTGGCGCGTAGCCAGGCCCCGGTC
TATATCAGCGGCGAGTCCGGCAGTGGCAAGGAGTTGGTGGCACGACTGATCCACGAACAGGGGCCGCGCGGCGAAAAACC
CTTTGTGCCGGTCAACTGTGGTGCCATTCCGACTGACCTGATGGAAAGCGAATTTTTCGGGCATCGCAAAGGCAGCTTCA
CGGGCGCTCACGAAGACAAGCCCGGACTGTTTCAAGCCGCCCAGAACGGCACGCTGTTTCTGGACGAGGTCGCTGACCTG
CCGCTGGCCATGCAGGTGAAACTGCTGCGCGCGATACAGGAAAAGTCCATTCGCAGCGTCGGCGGCCAGCAGGAACAGGT
GGTGGATGTACGGATCCTCTGTGCGACCCACAAGGACCTCAGCGTGGAAGTGGCGGCCGGGCGCTTCCGTCAGGATCTCT
ATTACCGCTTGAACGTCATTGAGCTGTGCGTGCCTTCCCTGCGTGAGCGTCGCGAAGACATTGACCAACTCGCGGCCATC
GTCCTGCAACGCCTGGCAGCCAATAGCGGCCTACCTGCGGCCAGGCTGAATGCGCAGGCGCTGGAAACCCTGAAGAACTA
CCGTTTTCCCGGCAACGTCCGAGAGCTGGAAAACATGTTGGAACGGGCCTACACGCTGTGCGAGAACGATGAAATCCATA
CCAGCGACCTGCGCCTGGCGGAGTCGGTCAGAACCGCAGAAAGCGACGGCCCCAACCTGGCCGACATCGACAATCTGGAA
GATTATCTGGAAGGCATCGAACGCAAACTGATTCTGCAGGCCCTGGAAGAGACCCGCTGGAACCGGACAGCAGCAGCCCA
ACGACTCAGCCTGTCATTCAGGTCCATGCGCTATCGGTTAAAAAAGCTGGGGCTGGATTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-116)

Sigma-54 interaction domain (Sigma54_activat)

(137-302)

Bacterial regulatory protein, Fis family (HTH_8)

(403-443)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

80.135

99.327

0.796
  pilR Acinetobacter baumannii strain A118

49.02

100

0.504

Multiple sequence alignment