Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   N015_RS22415 Genome accession   NZ_CP047265
Coordinates   5030039..5031625 (+) Length   528 a.a.
NCBI ID   WP_032622576.1    Uniprot ID   -
Organism   Pseudomonas asturiensis strain CC1524     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5025039..5036625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N015_RS22395 (N015_22395) pgeF 5026830..5027558 (-) 729 WP_024685588.1 peptidoglycan editing factor PgeF -
  N015_RS22400 (N015_22400) rluD 5027555..5028517 (-) 963 WP_024685589.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  N015_RS22405 (N015_22405) - 5028667..5029689 (+) 1023 WP_024685590.1 outer membrane protein assembly factor BamD -
  N015_RS22410 (N015_22410) - 5029810..5030049 (+) 240 WP_080265980.1 PP0621 family protein -
  N015_RS22415 (N015_22415) pilS 5030039..5031625 (+) 1587 WP_032622576.1 sensor histidine kinase Regulator
  N015_RS22420 (N015_22420) pilR 5031634..5032974 (+) 1341 WP_024685591.1 sigma-54-dependent transcriptional regulator Regulator
  N015_RS22425 (N015_22425) - 5033171..5033386 (-) 216 Protein_4442 NAD(P)/FAD-dependent oxidoreductase -
  N015_RS22430 (N015_22430) - 5033618..5033836 (-) 219 Protein_4443 NAD(P)/FAD-dependent oxidoreductase -
  N015_RS22435 (N015_22435) - 5034065..5034280 (-) 216 Protein_4444 NAD(P)/FAD-dependent oxidoreductase -
  N015_RS22440 (N015_22440) thiO 5034508..5035620 (-) 1113 WP_159372212.1 glycine oxidase ThiO -
  N015_RS22445 (N015_22445) - 5035647..5036048 (-) 402 WP_024688922.1 type IV pilin protein -

Sequence

Protein


Download         Length: 528 a.a.        Molecular weight: 58023.94 Da        Isoelectric Point: 6.6658

>NTDB_id=411929 N015_RS22415 WP_032622576.1 5030039..5031625(+) (pilS) [Pseudomonas asturiensis strain CC1524]
MSAETLSLGSAQAHRILQLYHLYRLVIGILLVSLISARLDAELLQLADTDLFRAGCWLYLVSNILIVVLLEQPKRQAHLF
GLAMADALMLSGLFYAAGGPSSGIGNLLIASVAISNILLRGRSGLLIAAVSAIAIIYLTFYISFSTPAVANDYVQAGSLG
TLCFAAAMLVQALTARMQVSEVLAKQRAADVDNLEELNALILQRMRTGILVLDARRQVLLANQGALNLLGHEQLVGQVID
SYSPELVDRFRHWLINPIMVPRSISTSAIGPVLQPSFAALNRGDQRQTLIFLEDLSQIAQQAQQLKLAALGRLTAGIAHE
IRNPLGAVSHAAQLLNESEELTAPDRRLGQIIHDQSRRINVVIENVLQLSRRREAHPELLDLRQWLDTFVTDFRTSAAPD
QQLHIDIGSGMLTTRMDPEQLTQVVTNLLQNGLRYSGQQHPIAQVWLRLYHALQSNLPTLEVLDDGAGVSENHLTRLFEP
FFTTESKGTGLGLYLSRELCESNQAHLDYAPREGGGSCLRITFAHPFK

Nucleotide


Download         Length: 1587 bp        

>NTDB_id=411929 N015_RS22415 WP_032622576.1 5030039..5031625(+) (pilS) [Pseudomonas asturiensis strain CC1524]
GTGAGCGCTGAGACGCTCTCACTGGGCAGCGCCCAAGCGCATCGTATCCTGCAGCTCTATCACCTTTATCGCCTTGTCAT
TGGGATCCTGCTGGTATCGCTGATCTCTGCCCGCCTGGACGCCGAACTGCTGCAACTGGCCGATACCGACCTGTTCCGGG
CAGGTTGCTGGCTGTATCTGGTATCAAACATTCTCATCGTGGTGCTGCTCGAACAGCCCAAACGTCAGGCTCACCTGTTC
GGCCTGGCGATGGCGGATGCGCTCATGTTGTCAGGGCTGTTCTATGCGGCAGGCGGCCCGTCGAGCGGTATCGGCAACCT
GCTGATCGCGTCAGTGGCCATCAGCAACATCCTGTTGCGTGGCCGCAGCGGCCTGCTGATTGCCGCGGTCTCGGCCATCG
CCATTATCTACCTGACCTTCTATATCAGCTTCAGCACCCCTGCGGTCGCCAACGACTACGTGCAGGCGGGCTCGCTGGGC
ACGTTGTGTTTTGCTGCGGCCATGCTGGTGCAGGCACTCACGGCGCGCATGCAGGTCAGCGAGGTACTGGCCAAACAGCG
CGCTGCCGATGTCGATAATCTTGAAGAGCTCAATGCCCTGATTCTGCAGCGCATGCGCACCGGCATTCTGGTGCTGGACG
CGCGACGCCAGGTGCTGCTGGCCAACCAGGGCGCACTGAACCTGTTGGGTCATGAGCAATTGGTCGGCCAGGTGATCGAC
AGCTATTCACCTGAGCTGGTGGACCGTTTTCGTCATTGGCTGATCAATCCGATCATGGTGCCGCGCAGCATCTCGACCTC
GGCGATCGGCCCTGTGCTGCAACCCAGCTTCGCCGCACTGAACCGAGGTGATCAGCGCCAGACCCTGATTTTTCTGGAGG
ACCTGTCACAGATTGCCCAGCAGGCTCAGCAACTCAAGCTGGCCGCACTGGGTCGCCTGACTGCCGGGATCGCCCATGAA
ATCCGCAATCCCCTGGGCGCGGTCAGCCACGCAGCGCAGTTGTTGAACGAATCGGAAGAACTGACTGCGCCGGATCGGCG
ACTGGGACAGATCATCCATGACCAGTCACGGCGCATCAATGTGGTGATCGAGAACGTACTGCAACTGTCACGACGACGCG
AAGCCCACCCCGAACTGCTCGACCTGAGGCAGTGGCTCGACACGTTCGTTACAGACTTTCGCACCTCTGCCGCACCCGAT
CAGCAGCTTCACATCGATATCGGCAGCGGCATGCTGACCACCCGCATGGACCCGGAACAGTTGACTCAGGTCGTGACCAA
CCTGCTGCAGAACGGGCTGCGCTACAGCGGTCAGCAGCACCCCATCGCCCAGGTATGGCTCAGGCTGTATCACGCCCTTC
AGAGCAACTTGCCGACACTTGAAGTTCTGGACGATGGTGCGGGCGTCAGTGAGAACCACCTGACCCGCCTGTTCGAACCC
TTCTTCACCACCGAAAGCAAAGGGACAGGTCTGGGCCTCTACCTGTCACGCGAGCTATGCGAAAGCAATCAGGCGCACCT
GGACTACGCCCCTCGGGAAGGCGGCGGCAGTTGTCTGCGCATTACCTTCGCCCACCCGTTCAAATAG

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

60.606

100

0.606

Multiple sequence alignment