Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N015_RS22310 Genome accession   NZ_CP047265
Coordinates   5009636..5011330 (+) Length   564 a.a.
NCBI ID   WP_024684954.1    Uniprot ID   -
Organism   Pseudomonas asturiensis strain CC1524     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5004636..5016330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N015_RS22280 (N015_22280) - 5004742..5005875 (-) 1134 WP_024684949.1 phosphoribosyltransferase domain-containing protein -
  N015_RS22285 (N015_22285) - 5005954..5007096 (-) 1143 WP_024684950.1 trypsin-like peptidase domain-containing protein -
  N015_RS22290 (N015_22290) - 5007151..5008149 (-) 999 WP_024684951.1 ATP-grasp domain-containing protein -
  N015_RS22295 (N015_22295) - 5008269..5008814 (+) 546 WP_024684952.1 DUF1523 family protein -
  N015_RS22305 (N015_22305) - 5008987..5009409 (-) 423 WP_024684953.1 pilin -
  N015_RS22310 (N015_22310) pilB 5009636..5011330 (+) 1695 WP_024684954.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N015_RS22315 (N015_22315) pilC 5011333..5012550 (+) 1218 WP_024684955.1 type II secretion system F family protein Machinery gene
  N015_RS22320 (N015_22320) pilD 5012552..5013424 (+) 873 WP_024684956.1 prepilin peptidase Machinery gene
  N015_RS22325 (N015_22325) coaE 5013421..5014044 (+) 624 WP_024684957.1 dephospho-CoA kinase -
  N015_RS22330 (N015_22330) yacG 5014041..5014250 (+) 210 WP_024684958.1 DNA gyrase inhibitor YacG -
  N015_RS22335 (N015_22335) - 5014494..5014955 (-) 462 WP_024685627.1 hypothetical protein -
  N015_RS22340 (N015_22340) - 5015081..5015710 (+) 630 WP_024685626.1 DUF1780 domain-containing protein -
  N015_RS22345 (N015_22345) - 5015707..5016240 (+) 534 WP_024685625.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62256.50 Da        Isoelectric Point: 6.3852

>NTDB_id=411926 N015_RS22310 WP_024684954.1 5009636..5011330(+) (pilB) [Pseudomonas asturiensis strain CC1524]
MSDVVLTGLAKQLVLAELLSEKAAQQANQNAKRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
ISEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLSDAIDKFFDANSGLGDLADVDLGLD
IEIGDGKESSLSEQSDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKVFRVRLRTDGILHEVARPPIHLASRIAARLKV
MASLDISERRKPQDGRVKLRISKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDVPRETLLQEGFPESK
IGTFKIYGPVGCNHCNGGYKGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=411926 N015_RS22310 WP_024684954.1 5009636..5011330(+) (pilB) [Pseudomonas asturiensis strain CC1524]
ATGTCTGATGTCGTTCTCACGGGGCTTGCCAAGCAACTGGTGCTGGCCGAGCTTCTTTCTGAAAAAGCTGCACAGCAGGC
TAACCAAAACGCCAAGCGCGACAAGATTTCGTTGGTCAGCTACCTGGTTCAGAACAAGCTGGTCAAAAGTCTGACGCTTG
CCGAGATGGCCTCCGATCAATTCGGCGTGCCGTTCATGGACCTTGCCAGCCTGGACAAGGAAAGTCAGCCCAAAGGGTTG
ATCAGTGAAAAGCTGGTTCGCCAGCACCATGCATTGCCGTTATGGCGTCGGGGCAACAAGCTGTTCATCGGCATTTCAGA
CCCGACCAACCATCAGGCCGTCACCGACATTCAGTTCAGCACCGGCCTCAACACCGAAGCGATTCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGACAAGTTCTTCGACGCCAATAGCGGCCTGGGCGATCTGGCCGATGTCGACCTCGGGCTGGAC
ATCGAAATAGGCGATGGCAAGGAGTCGTCTCTCAGCGAACAGAGCGATGCCGATGACGCGCCGGTGGTGCGCTTCGTCAA
CAAGATGCTGATGGACGCGATCCGGCTGGGCTCTTCGGACCTGCACTTCGAACCCTACGAGAAAGTATTTCGCGTGCGCC
TGCGCACCGACGGCATCCTGCATGAAGTCGCCCGGCCGCCGATTCACCTCGCCAGCCGAATCGCCGCGCGTCTGAAGGTC
ATGGCCAGCCTGGACATTTCCGAACGACGCAAGCCTCAGGACGGACGGGTCAAGCTGCGCATCTCCAAGACCAAGGCCAT
CGATTTTCGTATGAACACCCTGCCGACGCTGTGGGGCGAGAAGATCGTCATGCGGATTCTCGATCCGACCAGCGCCCAGA
TGGGCATCGATGCCCTGGGCTACGAGCCTGAACAGAAAGCGCTGTATCTGGAAGCGTTGAAGCAGCCACAGGGGATGATT
CTGGTCACAGGCCCCACCGGCTCCGGCAAGACCGTGTCGCTGTACACAGGCCTGAACATCCTCAATACGGTGGACATCAA
CATTTCTACCGCCGAAGACCCGGTCGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGTCAGGGCATGG
ACTTCTCTCAGGCGCTGCGCGCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTGGAAACG
GCAGAAATCGCCATCAAGGCCTCGCAGACCGGTCACATGGTCTTGTCCACGCTGCACACCAACAGCGCGGCGGAAACCCT
GACTCGTTTGCACCACATGGGGGTCGCAGCCTTCAACATTGCCACGGCGATCAACCTGATCATCGCGCAACGTCTGGCTC
GCAAGCTATGCTCCCACTGCAAAAAAGAGCTGGATGTTCCCCGCGAAACACTGCTTCAGGAAGGTTTTCCCGAGTCAAAA
ATAGGCACGTTCAAGATCTACGGCCCGGTTGGCTGTAATCACTGCAACGGCGGGTACAAGGGGCGGGTCGGCATTTACGA
AGTCGTCAAGAAAACGCCTGAGCTGGAGCGCATCATCATGGAAGAAGGCAATTCGCTGGAAATTTCCAGGCAAATGCGCA
AGGACGGCTTCAATGACCTGCGCACTTCAGGCCTTTCGAAAGCCATGCAGGGCATTACCAGCCTGGAAGAAGTCAATCGC
GTGACCAAGGATTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.007

99.645

0.578
  pilB Acinetobacter baylyi ADP1

57.651

99.645

0.574
  pilB Legionella pneumophila strain ERS1305867

54.947

100

0.551
  pilB Vibrio cholerae strain A1552

51.332

99.823

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

51.296

95.745

0.491
  pilB Vibrio campbellii strain DS40M4

48.936

100

0.489
  pilF Neisseria gonorrhoeae MS11

47.687

99.645

0.475
  pilF Thermus thermophilus HB27

37.809

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.686

97.163

0.376

Multiple sequence alignment