Detailed information NTG0411884

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PMA4326_RS01485
• Genome accession: NZ_CP047260
• Coordinates: 302215..303279 (+)
• Length: 354 a.a.
• NCBI ID: WP_007248061.1
• Uniprot ID: A0A3M3ZTK3
• Organism: Pseudomonas syringae pv. maculicola str. ES4326
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 297215..308279

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PMA4326_RS01470 (PMA4326_001470)	-	298106..299374 (+)	1269	WP_007248063.1	malic enzyme-like NAD(P)-binding protein	-
PMA4326_RS01480 (PMA4326_001480)	-	299561..302008 (-)	2448	WP_007248062.1	penicillin-binding protein 1A	-
PMA4326_RS01485 (PMA4326_001485)	pilM	302215..303279 (+)	1065	WP_007248061.1	pilus assembly protein PilM	Machinery gene
PMA4326_RS01490 (PMA4326_001490)	-	303279..303854 (+)	576	WP_007248060.1	PilN domain-containing protein	-
PMA4326_RS01495 (PMA4326_001495)	pilO	303851..304474 (+)	624	WP_007248059.1	type 4a pilus biogenesis protein PilO	-
PMA4326_RS01500 (PMA4326_001500)	pilP	304471..304998 (+)	528	WP_007248058.1	type 4a pilus biogenesis lipoprotein PilP	-
PMA4326_RS01505 (PMA4326_001505)	pilQ	305046..307124 (+)	2079	WP_007248057.1	type IV pilus secretin PilQ	Machinery gene
PMA4326_RS01510 (PMA4326_001510)	aroK	307129..307647 (+)	519	WP_007248056.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38106.62 Da
• Isoelectric Point: 4.7008

>NTDB_id=411884 PMA4326_RS01485 WP_007248061.1 302215..303279(+) (pilM) [Pseudomonas syringae pv. maculicola str. ES4326]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=411884 PMA4326_RS01485 WP_007248061.1 302215..303279(+) (pilM) [Pseudomonas syringae pv. maculicola str. ES4326]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCCTACGCAGTCGAGCCGTTGCCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCTGAACTCGAAGGGGTTGGCCAGGCGTTGTCTCGCGTGCTGGTCAAGGCCAAGACCAGCGTCAAGATTGTGGCGGTT
GCAGTGGCCGGGTCCGCAGTCATCACCAAAACCATCGAGATGGACGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATTCCCTATCCGCTTGAAGAAGTGGCCATCGATTTTGAAGTGCAGGGCTATTCGGTAC
GCAATCCGGAACGGGTTGAAGTGCTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCGGCGCTGGCGCTG
GCCGGGCTGACCGCGCGCGTTGTTGACGTCGAAGCCTATGCGTTGGAGCGCTCGTTCGGCCTGCTGGCTGCGCAATTGGG
GAACGGCCACGACGAGCTGACCGTTGCGGTGGTCGATATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACCATGGGC
GGATCATCTATACCCGCGAGCAACTGTTCGGCGGCCGCCAGCTGACCGACGAGATTCAGCGCCGCTATGGCCTGTCCATG
GAAGAGGCCGGGCTTGCCAAGAAACAGGGCGGGCTGCCTGACGACTATGTCAGCGAGGTGCTGGACCCGTTCAAGGACGC
GCTGGTGCAGCAGGTTTCCCGCTCGTTGCAGTTCTTCTTTGCGGCGGGGCAGTACAACTCGGTGGATCACATCATGCTGG
CAGGCGGTACGGCTTCGATTTCCGGGCTGGAGCACCTGATTCAGCGACGTATCGGCACGCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGTTCCAAGGTCAATGCGGGCGCACTGGCCAGTGACGCCCCGGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A3M3ZTK3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469