Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GRT08_RS12010 Genome accession   NZ_CP047234
Coordinates   2475265..2476335 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain FJ4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2470265..2481335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRT08_RS11960 (GRT08_11790) sipW 2470325..2470909 (-) 585 WP_060562614.1 signal peptidase I SipW -
  GRT08_RS11965 (GRT08_11795) tapA 2470881..2471552 (-) 672 WP_071391613.1 amyloid fiber anchoring/assembly protein TapA -
  GRT08_RS11970 (GRT08_11800) - 2471811..2472140 (+) 330 WP_071391612.1 DUF3889 domain-containing protein -
  GRT08_RS11975 (GRT08_11805) - 2472180..2472359 (-) 180 WP_003153093.1 YqzE family protein -
  GRT08_RS11980 (GRT08_11810) comGG 2472416..2472793 (-) 378 WP_071391611.1 competence type IV pilus minor pilin ComGG Machinery gene
  GRT08_RS11985 (GRT08_11815) comGF 2472794..2473189 (-) 396 WP_071391610.1 competence type IV pilus minor pilin ComGF -
  GRT08_RS11990 (GRT08_11820) comGE 2473203..2473517 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  GRT08_RS11995 (GRT08_11825) comGD 2473501..2473938 (-) 438 WP_025852922.1 competence type IV pilus minor pilin ComGD Machinery gene
  GRT08_RS12000 (GRT08_11830) comGC 2473928..2474236 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  GRT08_RS12005 (GRT08_11835) comGB 2474241..2475278 (-) 1038 WP_063174751.1 competence type IV pilus assembly protein ComGB Machinery gene
  GRT08_RS12010 (GRT08_11840) comGA 2475265..2476335 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  GRT08_RS12015 (GRT08_11845) - 2476526..2477476 (-) 951 WP_071391609.1 magnesium transporter CorA family protein -
  GRT08_RS12020 (GRT08_11850) - 2477622..2478923 (+) 1302 WP_070082114.1 hemolysin family protein -
  GRT08_RS12025 (GRT08_11855) - 2478961..2479791 (-) 831 WP_071391608.1 STAS domain-containing protein -
  GRT08_RS12035 (GRT08_11865) - 2479996..2480376 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  GRT08_RS12040 (GRT08_11870) - 2480613..2480858 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=411692 GRT08_RS12010 WP_003153083.1 2475265..2476335(-) (comGA) [Bacillus amyloliquefaciens strain FJ4]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=411692 GRT08_RS12010 WP_003153083.1 2475265..2476335(-) (comGA) [Bacillus amyloliquefaciens strain FJ4]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTCTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC
ACAAGGGATGCTAAGGGCGCCATATACAGACTGTTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTTACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment