Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   GRT08_RS12000 Genome accession   NZ_CP047234
Coordinates   2473928..2474236 (-) Length   102 a.a.
NCBI ID   WP_003153087.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain FJ4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2468928..2479236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRT08_RS11950 (GRT08_11780) sinR 2469093..2469428 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  GRT08_RS11955 (GRT08_11785) tasA 2469476..2470261 (-) 786 WP_007408329.1 biofilm matrix protein TasA -
  GRT08_RS11960 (GRT08_11790) sipW 2470325..2470909 (-) 585 WP_060562614.1 signal peptidase I SipW -
  GRT08_RS11965 (GRT08_11795) tapA 2470881..2471552 (-) 672 WP_071391613.1 amyloid fiber anchoring/assembly protein TapA -
  GRT08_RS11970 (GRT08_11800) - 2471811..2472140 (+) 330 WP_071391612.1 DUF3889 domain-containing protein -
  GRT08_RS11975 (GRT08_11805) - 2472180..2472359 (-) 180 WP_003153093.1 YqzE family protein -
  GRT08_RS11980 (GRT08_11810) comGG 2472416..2472793 (-) 378 WP_071391611.1 competence type IV pilus minor pilin ComGG Machinery gene
  GRT08_RS11985 (GRT08_11815) comGF 2472794..2473189 (-) 396 WP_071391610.1 competence type IV pilus minor pilin ComGF -
  GRT08_RS11990 (GRT08_11820) comGE 2473203..2473517 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  GRT08_RS11995 (GRT08_11825) comGD 2473501..2473938 (-) 438 WP_025852922.1 competence type IV pilus minor pilin ComGD Machinery gene
  GRT08_RS12000 (GRT08_11830) comGC 2473928..2474236 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  GRT08_RS12005 (GRT08_11835) comGB 2474241..2475278 (-) 1038 WP_063174751.1 competence type IV pilus assembly protein ComGB Machinery gene
  GRT08_RS12010 (GRT08_11840) comGA 2475265..2476335 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  GRT08_RS12015 (GRT08_11845) - 2476526..2477476 (-) 951 WP_071391609.1 magnesium transporter CorA family protein -
  GRT08_RS12020 (GRT08_11850) - 2477622..2478923 (+) 1302 WP_070082114.1 hemolysin family protein -

Sequence

Protein


Download         Length: 102 a.a.        Molecular weight: 11381.28 Da        Isoelectric Point: 6.2096

>NTDB_id=411690 GRT08_RS12000 WP_003153087.1 2473928..2474236(-) (comGC) [Bacillus amyloliquefaciens strain FJ4]
MLRLKNQDGFTLIEMLIVLFIVSILLLITIPNVTKHNQSIQHKGCEGLQNMVKAQVTAYEIDHEGKMPDMNDLQSEGYIK
KNTACPNGKQILISGGEVTVEQ

Nucleotide


Download         Length: 309 bp        

>NTDB_id=411690 GRT08_RS12000 WP_003153087.1 2473928..2474236(-) (comGC) [Bacillus amyloliquefaciens strain FJ4]
GTGCTTCGTCTGAAAAATCAAGATGGATTTACTTTAATTGAAATGCTGATCGTTTTATTTATCGTTTCCATTCTGCTTTT
AATCACCATTCCTAACGTTACAAAACATAATCAAAGCATTCAGCATAAAGGCTGTGAAGGACTGCAGAATATGGTGAAAG
CCCAAGTGACGGCGTACGAAATCGACCATGAAGGTAAAATGCCGGATATGAACGATTTACAATCAGAGGGGTATATCAAA
AAGAATACAGCCTGCCCGAATGGTAAACAGATCTTAATTAGTGGCGGAGAAGTTACAGTTGAACAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

77.083

94.118

0.725
  comGC Staphylococcus aureus MW2

42.708

94.118

0.402
  comGC Staphylococcus aureus N315

42.708

94.118

0.402

Multiple sequence alignment